St. Ambrose Church, Saint Ambrose Parish, Église Saint-Ambroise, or variations, may refer to:
- Saint-Ambroise Church, Montreal, Canada
- Église Saint-Ambroise, Paris, France
- St. Ambrose Church (West Hollywood, California)
- Saint Ambrose Church (Bridgeport, Connecticut)
- St. Ambrose Cathedral (Des Moines, Iowa)
- Saint Ambrose Church (Cheverly, Maryland)
- St. Ambrose's Church (New York City)
- St. Ambrose Church (St. Nazianz, Wisconsin)
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