This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology.

Contents 1 EC 3.1: Acting on Ester Bonds 1.1 EC 3.1.1: Carboxylic Ester Hydrolases 1.2 EC 3.1.2: Thioester Hydrolases 1.3 EC 3.1.3: Phosphoric Monoester Hydrolases 1.4 EC 3.1.4: Phosphoric Diester Hydrolases 1.5 EC 3.1.5: Triphosphoric Monoester Hydrolases 1.6 EC 3.1.6: Sulfuric Ester Hydrolases 1.7 EC 3.1.7: Diphosphoric Monoester Hydrolases 1.8 EC 3.1.8: Phosphoric Triester Hydrolases 1.9 EC 3.1.11: Exodeoxyribonucleases Producing 5'-Phosphomonoesters 1.10 EC 3.1.13: Exoribonucleases Producing 5'-Phosphomonoesters 1.11 EC 3.1.14: Exoribonucleases Producing 3'-Phosphomonoesters 1.12 EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters 1.13 EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters 1.14 EC 3.1.21: Endodeoxyribonucleases Producing 5'-Phosphomonoesters 1.15 EC 3.1.22: Endodeoxyribonucleases Producing 3'-Phosphomonoesters 1.16 EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5 1.17 EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases 1.18 EC 3.1.26: Endoribonucleases Producing 5'-Phosphomonoesters 1.19 EC 3.1.27: Endoribonucleases Producing 3'-Phosphomonoesters 1.20 EC 3.1.30: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters 1.21 EC 3.1.31: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters 2 EC 3.2: Glycosylases 2.1 EC 3.2.1: Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds 2.2 EC 3.2.2: Hydrolysing N-Glycosyl Compounds 2.3 EC 3.2.3: Hydrolysing S-Glycosyl Compounds 3 EC 3.3: Acting on Ether Bonds 3.1 EC 3.3.1: Thioether and trialkylsulfonium hydrolases 3.2 EC 3.3.2: Ether Hydrolases 4 EC 3.4: act on peptide bonds - Peptidase 4.1 EC 3.4.1 α-amino acyl peptide hydrolases (discontinued) 4.2 EC 3.4.2 Peptidyl amino acid hydrolases (discontinued) 4.3 EC 3.4.3 Dipeptide Hydrolases (discontinued) 4.4 EC 3.4.4 Peptidyl Peptide Hydrolases (discontinued) 4.5 EC 3.4.11 Aminopeptidases 4.6 EC 3.4.13 Dipeptidases 4.7 EC 3.4.14 Dipeptidyl peptidases and tripeptidyl peptidases 4.8 EC 3.4.15 Peptidyl dipeptidases 4.9 EC 3.4.16 Serine type carboxypeptidases 4.10 EC 3.4.17 Metallocarboxypeptidases 4.11 EC 3.4.18 Cysteine type carboxypeptidases 4.12 EC 3.4.19 Omega peptidases 4.13 EC 3.4.21: Serine proteases 4.14 EC 3.4.22 Cysteine proteases 4.15 EC 3.4.23 Aspartic endopeptidases 4.16 EC 3.4.24: Metallopeptidases 4.17 EC 3.4.25 Threonine endopeptidases 4.18 EC 3.4.99 Endopeptidases of unknown catalytic mechanism 5 EC 3.5: act on carbon-nitrogen bonds, other than peptide bonds 5.1 3.5.1: linear amides 5.2 3.5.2: cyclic amides 5.3 3.5.3: linear amidines 5.4 3.5.4: cyclic amidines 5.5 3.5.5: nitriles 5.6 3.5.99: other compounds 6 EC 3.6: act on acid anhydrides 6.1 3.6.1 6.2 3.6.2 6.3 3.6.3 6.4 3.6.4 6.5 3.6.5 7 EC 3.7: act on carbon-carbon bonds 8 EC 3.8: act on halide bonds 9 EC 3.9: act on phosphorus-nitrogen bonds 10 EC 3.10: act on sulfur-nitrogen bonds 11 EC 3.11: act on carbon-phosphorus bonds 12 EC 3.12: act on sulfur-sulfur bonds 13 EC 3.13: act on carbon-sulfur bonds

EC 3.1: Acting on Ester Bonds[edit]

EC 3.1.1: Carboxylic Ester Hydrolases[edit]

• EC 3.1.1.1: carboxylesterase
• EC 3.1.1.2: arylesterase
• EC 3.1.1.3: triacylglycerol lipase
• EC 3.1.1.4: phospholipase A2
• EC 3.1.1.5: lysophospholipase
• EC 3.1.1.6: acetylesterase
• EC 3.1.1.7: acetylcholinesterase
• EC 3.1.1.8: cholinesterase
• EC 3.1.1.9: deleted
• EC 3.1.1.10: tropinesterase
• EC 3.1.1.11: pectinesterase
• EC 3.1.1.12: deleted
• EC 3.1.1.13: sterol esterase
• EC 3.1.1.14: chlorophyllase
• EC 3.1.1.15: L-arabinonolactonase
• EC 3.1.1.16: deleted, mixture of EC 5.3.3.4 and EC 3.1.1.24
• EC 3.1.1.17: gluconolactonase
• EC 3.1.1.18: deleted, included in EC 3.1.1.17
• EC 3.1.1.19: uronolactonase
• EC 3.1.1.20: tannase
• EC 3.1.1.21: retinyl-palmitate esterase
• EC 3.1.1.22: hydroxybutyrate-dimer hydrolase
• EC 3.1.1.23: acylglycerol lipase
• EC 3.1.1.24: 3-oxoadipate enol-lactonase
• EC 3.1.1.25: 1,4-lactonase
• EC 3.1.1.26: galactolipase
• EC 3.1.1.27: 4-pyridoxolactonase
• EC 3.1.1.28: acylcarnitine hydrolase
• EC 3.1.1.29: aminoacyl-tRNA hydrolase
• EC 3.1.1.30: D-arabinonolactonase
• EC 3.1.1.31: 6-phosphogluconolactonase
• EC 3.1.1.32: phospholipase A1
• EC 3.1.1.33: 6-acetylglucose deacetylase
• EC 3.1.1.34: lipoprotein lipase
• EC 3.1.1.35: dihydrocoumarin hydrolase
• EC 3.1.1.36: limonin-D-ring-lactonase
• EC 3.1.1.37: steroid-lactonase
• EC 3.1.1.38: triacetate-lactonase
• EC 3.1.1.39: actinomycin lactonase
• EC 3.1.1.40: orsellinate-depside hydrolase
• EC 3.1.1.41: cephalosporin-C deacetylase
• EC 3.1.1.42: chlorogenate hydrolase
• EC 3.1.1.43: a-amino-acid esterase
• EC 3.1.1.44: 4-methyloxaloacetate esterase
• EC 3.1.1.45: carboxymethylenebutenolidase
• EC 3.1.1.46: deoxylimonate A-ring-lactonase
• EC 3.1.1.47: 1-alkyl-2-acetylglycerophosphocholine esterase
• EC 3.1.1.48: fusarinine-C ornithinesterase
• EC 3.1.1.49: sinapine esterase
• EC 3.1.1.50: wax-ester hydrolase
• EC 3.1.1.51: phorbol-diester hydrolase
• EC 3.1.1.52: phosphatidylinositol deacylase
• EC 3.1.1.53: sialate O-acetylesterase
• EC 3.1.1.54: acetoxybutynylbithiophene deacetylase
• EC 3.1.1.55: acetylsalicylate deacetylase
• EC 3.1.1.56: methylumbelliferyl-acetate deacetylase
• EC 3.1.1.57: 2-pyrone-4,6-dicarboxylate lactonase
• EC 3.1.1.58: N-acetylgalactosaminoglycan deacetylase
• EC 3.1.1.59: juvenile-hormone esterase
• EC 3.1.1.60: bis(2-ethylhexyl)phthalate esterase
• EC 3.1.1.61: protein-glutamate methylesterase
• EC 3.1.1.62: now EC 3.5.1.47
• EC 3.1.1.63: 11-cis-retinyl-palmitate hydrolase
• EC 3.1.1.64: all-trans-retinyl-palmitate hydrolase
• EC 3.1.1.65: L-rhamnono-1,4-lactonase
• EC 3.1.1.66: 5-(3,4-diacetoxybut-1-ynyl)-2,2'-bithiophene deacetylase
• EC 3.1.1.67: fatty-acyl-ethyl-ester synthase
• EC 3.1.1.68: xylono-1,4-lactonase
• EC 3.1.1.69: now EC 3.5.1.89
• EC 3.1.1.70: cetraxate benzylesterase
• EC 3.1.1.71: acetylalkylglycerol acetylhydrolase
• EC 3.1.1.72: acetylxylan esterase
• EC 3.1.1.73: feruloyl esterase
• EC 3.1.1.74: cutinase
• EC 3.1.1.75: poly(3-hydroxybutyrate) depolymerase
• EC 3.1.1.76: poly(3-hydroxyoctanoate) depolymerase
• EC 3.1.1.77: acyloxyacyl hydrolase
• EC 3.1.1.78: polyneuridine-aldehyde esterase
• EC 3.1.1.79: hormone-sensitive lipase
• EC 3.1.1.80: acetylajmaline esterase
• EC 3.1.1.81: quorum-quenching N-acyl-homoserine lactonase
• EC 3.1.1.82: pheophorbidase
• EC 3.1.1.83: monoterpene epsilon-lactone hydrolase
• EC 3.1.1.84: cocaine esterase
• EC 3.1.1.85: pimelyl-(acyl-carrier protein) methyl ester esterase
• EC 3.1.1.86: rhamnogalacturonan acetylesterase
• EC 3.1.1.87: fumonisin B1 esterase
• EC 3.1.1.88: pyrethroid hydrolase
• EC 3.1.1.89: protein phosphatase methylesterase-1
• EC 3.1.1.90: all-trans-retinyl ester 13-cis isomerohydrolase
• EC 3.1.1.91: 2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase
• EC 3.1.1.92: 4-sulfomuconolactone hydrolase
• EC 3.1.1.93: mycophenolic acid acyl-glucuronide esterase

EC 3.1.2: Thioester Hydrolases[edit]

• EC 3.1.2.1: acetyl-CoA hydrolase
• EC 3.1.2.2: palmitoyl-CoA hydrolase
• EC 3.1.2.3: succinyl-CoA hydrolase
• EC 3.1.2.4: 3-hydroxyisobutyryl-CoA hydrolase
• EC 3.1.2.5: hydroxymethylglutaryl-CoA hydrolase
• EC 3.1.2.6: hydroxyacylglutathione hydrolase
• EC 3.1.2.7: glutathione thiolesterase
• EC 3.1.2.8: deleted, included in EC 3.1.2.6
• EC 3.1.2.9: deleted
• EC 3.1.2.10: formyl-CoA hydrolase
• EC 3.1.2.11: acetoacetyl-CoA hydrolase
• EC 3.1.2.12: S-formylglutathione hydrolase
• EC 3.1.2.13: S-succinylglutathione hydrolase
• EC 3.1.2.14: oleoyl-(acyl-carrier-protein) hydrolase
• EC 3.1.2.15: ubiquitin thiolesterase
• EC 3.1.2.16: citrate lyase deacetylase
• EC 3.1.2.17: (S)-methylmalonyl-CoA hydrolase
• EC 3.1.2.18: ADP-dependent short-chain-acyl-CoA hydrolase
• EC 3.1.2.19: ADP-dependent medium-chain-acyl-CoA hydrolase
• EC 3.1.2.20: acyl-CoA hydrolase
• EC 3.1.2.21: Dodecanoyl-(acyl-carrier-protein) hydrolase
• EC 3.1.2.22: palmitoyl(protein) hydrolase
• EC 3.1.2.23: 4-hydroxybenzoyl-CoA thioesterase
• EC 3.1.2.24: transferred entry now EC 3.13.1.3
• EC 3.1.2.25: phenylacetyl-CoA hydrolase
• EC 3.1.2.26: bile-acid-CoA hydrolase
• EC 3.1.2.27: choloyl-CoA hydrolase
• EC 3.1.2.28: 1,4-dihydroxy-2-naphthoyl-CoA hydrolase
• EC 3.1.2.29: fluoroacetyl-CoA thioesterase

EC 3.1.3: Phosphoric Monoester Hydrolases[edit]

• EC 3.1.3.1: alkaline phosphatase
• EC 3.1.3.2: acid phosphatase
• EC 3.1.3.3: phosphoserine phosphatase
• EC 3.1.3.4: phosphatidate phosphatase
• EC 3.1.3.5: 5'-nucleotidase
• EC 3.1.3.6: 3'-nucleotidase
• EC 3.1.3.7: 3'(2'),5'-bisphosphate nucleotidase
• EC 3.1.3.8: 3-phytase
• EC 3.1.3.9: glucose-6-phosphatase
• EC 3.1.3.10: glucose-1-phosphatase
• EC 3.1.3.11: fructose-bisphosphatase
• EC 3.1.3.12: trehalose-phosphatase
• EC 3.1.3.13: bisphosphoglycerate phosphatase
• EC 3.1.3.14: methylphosphothioglycerate phosphatase
• EC 3.1.3.15: histidinol-phosphatase
• EC 3.1.3.16: phosphoprotein phosphatase
• EC 3.1.3.17: (phosphorylase) phosphatase
• EC 3.1.3.18: phosphoglycolate phosphatase
• EC 3.1.3.19: glycerol-2-phosphatase
• EC 3.1.3.20: phosphoglycerate phosphatase
• EC 3.1.3.21: glycerol-1-phosphatase
• EC 3.1.3.22: mannitol-1-phosphatase
• EC 3.1.3.23: sugar-phosphatase
• EC 3.1.3.24: sucrose-phosphatase
• EC 3.1.3.25: inositol-phosphate phosphatase
• EC 3.1.3.26: 4-phytase
• EC 3.1.3.27: phosphatidylglycerophosphatase
• EC 3.1.3.28: ADP-phosphoglycerate phosphatase
• EC 3.1.3.29: N-acylneuraminate-9-phosphatase
• EC 3.1.3.30: deleted, included in EC 3.1.3.31
• EC 3.1.3.31: nucleotidase
• EC 3.1.3.32: polynucleotide 3'-phosphatase
• EC 3.1.3.33: polynucleotide 5'-phosphatase
• EC 3.1.3.34: deoxynucleotide 3'-phosphatase
• EC 3.1.3.35: thymidylate 5'-phosphatase
• EC 3.1.3.36: phosphoinositide 5-phosphatase
• EC 3.1.3.37: sedoheptulose-bisphosphatase
• EC 3.1.3.38: 3-phosphoglycerate phosphatase
• EC 3.1.3.39: streptomycin-6-phosphatase
• EC 3.1.3.40: guanidinodeoxy-scyllo-inositol-4-phosphatase
• EC 3.1.3.41: 4-nitrophenylphosphatase
• EC 3.1.3.42: (glycogen-synthase-D) phosphatase
• EC 3.1.3.43: (pyruvate dehydrogenase (acetyl-transferring))-phosphatase
• EC 3.1.3.44: (acetyl-CoA carboxylase)-phosphatase
• EC 3.1.3.45: 3-deoxy-manno-octulosonate-8-phosphatase
• EC 3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase
• EC 3.1.3.47: (hydroxymethylglutaryl-CoA reductase (NADPH))-phosphatase
• EC 3.1.3.48: protein-tyrosine-phosphatase
• EC 3.1.3.49: (pyruvate kinase)-phosphatase
• EC 3.1.3.50: sorbitol-6-phosphatase
• EC 3.1.3.51: dolichyl-phosphatase
• EC 3.1.3.52: (3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring))-phosphatase
• EC 3.1.3.53: (myosin-light-chain) phosphatase
• EC 3.1.3.54: fructose-2,6-bisphosphate 6-phosphatase
• EC 3.1.3.55: caldesmon-phosphatase
• EC 3.1.3.56: inositol-polyphosphate 5-phosphatase
• EC 3.1.3.57: inositol-1,4-bisphosphate 1-phosphatase
• EC 3.1.3.58: sugar-terminal-phosphatase
• EC 3.1.3.59: alkylacetylglycerophosphatase
• EC 3.1.3.60: phosphoenolpyruvate phosphatase
• EC 3.1.3.61: deleted
• EC 3.1.3.62: multiple inositol-polyphosphate phosphatase
• EC 3.1.3.63: 2-carboxy-D-arabinitol-1-phosphatase
• EC 3.1.3.64: phosphatidylinositol-3-phosphatase
• EC 3.1.3.65: now with EC 3.1.3.64
• EC 3.1.3.66: phosphatidylinositol-3,4-bisphosphate 4-phosphatase
• EC 3.1.3.67: phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase
• EC 3.1.3.68: 2-deoxyglucose-6-phosphatase
• EC 3.1.3.69: glucosylglycerol 3-phosphatase
• EC 3.1.3.70: mannosyl-3-phosphoglycerate phosphatase
• EC 3.1.3.71: 2-phosphosulfolactate phosphatase
• EC 3.1.3.72: 5-phytase
• EC 3.1.3.73: a-ribazole phosphatase
• EC 3.1.3.74: pyridoxal phosphatase
• EC 3.1.3.75: phosphoethanolamine/phosphocholine phosphatase
• EC 3.1.3.76: lipid-phosphate phosphatase
• EC 3.1.3.77: acireductone synthase
• EC 3.1.3.78: phosphatidylinositol-4,5-bisphosphate 4-phosphatase
• EC 3.1.3.79: mannosylfructose-phosphate phosphatase
• EC 3.1.3.80: 2,3-bisphosphoglycerate 3-phosphatase
• EC 3.1.3.81: diacylglycerol diphosphate phosphatase
• EC 3.1.3.82: D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase
• EC 3.1.3.83: D-glycero-alpha-D-manno-heptose 1,7-bisphosphate 7-phosphatase
• EC 3.1.3.84: ADP-ribose 1-phosphate phosphatase
• EC 3.1.3.85: glucosyl-3-phosphoglycerate phosphatase
• EC 3.1.3.86: phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase
• EC 3.1.3.87: 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
• EC 3.1.3.88: 5-phosphoribostamycin phosphatase

EC 3.1.4: Phosphoric Diester Hydrolases[edit]

• EC 3.1.4.1: phosphodiesterase I
• EC 3.1.4.2: glycerophosphocholine phosphodiesterase
• EC 3.1.4.3: lecithinase C
• EC 3.1.4.4: phospholipase D
• EC 3.1.4.5: now EC 3.1.21.1
• EC 3.1.4.6: now EC 3.1.22.1
• EC 3.1.4.7: now EC 3.1.31.1
• EC 3.1.4.8: now EC 3.1.27.3
• EC 3.1.4.9: now EC 3.1.30.2
• EC 3.1.4.10: now EC 4.6.1.13
• EC 3.1.4.11: phosphoinositide phospholipase C
• EC 3.1.4.12: sphingomyelin phosphodiesterase
• EC 3.1.4.13: serine-ethanolaminephosphate phosphodiesterase
• EC 3.1.4.14: (acyl-carrier-protein) phosphodiesterase
• EC 3.1.4.15: adenylyl-(glutamate—ammonia ligase) hydrolase
• EC 3.1.4.16: 2',3'-cyclic-nucleotide 2'-phosphodiesterase
• EC 3.1.4.17: 3',5'-cyclic-nucleotide phosphodiesterase
• EC 3.1.4.18: now EC 3.1.16.1
• EC 3.1.4.19: now EC 3.1.13.3
• EC 3.1.4.20: now EC 3.1.13.1
• EC 3.1.4.21: now EC 3.1.30.1
• EC 3.1.4.22: now EC 3.1.27.5
• EC 3.1.4.23: now EC 3.1.27.1
• EC 3.1.4.24: deleted
• EC 3.1.4.25: now EC 3.1.11.1
• EC 3.1.4.26: deleted
• EC 3.1.4.27: now EC 3.1.11.2
• EC 3.1.4.28: now EC 3.1.11.3
• EC 3.1.4.29: deleted
• EC 3.1.4.30: now EC 3.1.21.2
• EC 3.1.4.31: now EC 3.1.11.4
• EC 3.1.4.32: deleted
• EC 3.1.4.33: deleted
• EC 3.1.4.34: deleted
• EC 3.1.4.35: 3',5'-cyclic-GMP phosphodiesterase
• EC 3.1.4.36: now with EC 3.1.4.43
• EC 3.1.4.37: 2',3'-cyclic-nucleotide 3'-phosphodiesterase
• EC 3.1.4.38: glycerophosphocholine cholinephosphodiesterase
• EC 3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase
• EC 3.1.4.40: CMP-N-acylneuraminate phosphodiesterase
• EC 3.1.4.41: sphingomyelin phosphodiesterase D
• EC 3.1.4.42: glycerol-1,2-cyclic-phosphate 2-phosphodiesterase
• EC 3.1.4.43: glycerophosphoinositol inositolphosphodiesterase
• EC 3.1.4.44: glycerophosphoinositol glycerophosphodiesterase
• EC 3.1.4.45: N-acetylglucosamine-1-phosphodiester a-N-acetylglucosaminidase
• EC 3.1.4.46: glycerophosphodiester phosphodiesterase
• EC 3.1.4.47: now EC 4.6.1.14
• EC 3.1.4.48: dolichylphosphate-glucose phosphodiesterase
• EC 3.1.4.49: dolichylphosphate-mannose phosphodiesterase
• EC 3.1.4.50: glycosylphosphatidylinositol phospholipase D
• EC 3.1.4.51: glucose-1-phospho-D-mannosylglycoprotein phosphodiesterase
• EC 3.1.4.52: cyclic-guanylate-specific phosphodiesterase
• EC 3.1.4.53: 3',5'-cyclic-AMP phosphodiesterase
• EC 3.1.4.54: N-acetylphosphatidylethanolamine-hydrolysing phospholipase D

EC 3.1.5: Triphosphoric Monoester Hydrolases[edit]

• EC 3.1.5.1: dGTPase

EC 3.1.6: Sulfuric Ester Hydrolases[edit]

• EC 3.1.6.1: arylsulfatase
• EC 3.1.6.2: steryl-sulfatase
• EC 3.1.6.3: glycosulfatase
• EC 3.1.6.4: N-acetylgalactosamine-6-sulfatase
• EC 3.1.6.5: deleted
• EC 3.1.6.6: choline-sulfatase
• EC 3.1.6.7: cellulose-polysulfatase
• EC 3.1.6.8: cerebroside-sulfatase
• EC 3.1.6.9: chondro-4-sulfatase
• EC 3.1.6.10: chondro-6-sulfatase
• EC 3.1.6.11: disulfoglucosamine-6-sulfatase
• EC 3.1.6.12: N-acetylgalactosamine-4-sulfatase
• EC 3.1.6.13: iduronate-2-sulfatase
• EC 3.1.6.14: N-acetylglucosamine-6-sulfatase
• EC 3.1.6.15: N-sulfoglucosamine-3-sulfatase
• EC 3.1.6.16: monomethyl-sulfatase
• EC 3.1.6.17: D-lactate-2-sulfatase
• EC 3.1.6.18: glucuronate-2-sulfatase

EC 3.1.7: Diphosphoric Monoester Hydrolases[edit]

• EC 3.1.7.1: prenyl-diphosphatase
• EC 3.1.7.2: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase
• EC 3.1.7.3: monoterpenyl-diphosphatase
• EC 3.1.7.4: sclareol cyclase
• EC 3.1.7.5: geranylgeranyl diphosphate diphosphatase
• EC 3.1.7.6: farnesyl diphosphatase
• EC 3.1.7.7: drimenol cyclase
• EC 3.1.7.8: tuberculosinol synthase
• EC 3.1.7.9: isotuberculosinol synthase
• EC 3.1.7.10: (13E)-labda-7,13-dien-15-ol synthase
• EC 3.1.7.11: geranyl diphosphate diphosphatase

EC 3.1.8: Phosphoric Triester Hydrolases[edit]

• EC 3.1.8.1: aryldialkylphosphatase
• EC 3.1.8.2: diisopropyl-fluorophosphatase

EC 3.1.11: Exodeoxyribonucleases Producing 5'-Phosphomonoesters[edit]

• EC 3.1.11.1: exodeoxyribonuclease I
• EC 3.1.11.2: exodeoxyribonuclease III
• EC 3.1.11.3: exodeoxyribonuclease (lambda-induced)
• EC 3.1.11.4: exodeoxyribonuclease (phage SP3-induced)
• EC 3.1.11.5: exodeoxyribonuclease V
• EC 3.1.11.6: exodeoxyribonuclease VII

EC 3.1.13: Exoribonucleases Producing 5'-Phosphomonoesters[edit]

• EC 3.1.13.1: exoribonuclease II
• EC 3.1.13.2: exoribonuclease H
• EC 3.1.13.3: oligonucleotidase
• EC 3.1.13.4: poly(A)-specific ribonuclease
• EC 3.1.13.5: ribonuclease D

EC 3.1.14: Exoribonucleases Producing 3'-Phosphomonoesters[edit]

• EC 3.1.14.1: yeast ribonuclease

EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters[edit]

• EC 3.1.15.1: venom exonuclease

EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters[edit]

• EC 3.1.16.1: spleen exonuclease

EC 3.1.21: Endodeoxyribonucleases Producing 5'-Phosphomonoesters[edit]

• EC 3.1.21.1: deoxyribonuclease I
• EC 3.1.21.2: deoxyribonuclease IV (phage-T4-induced)
• EC 3.1.21.3: type I site-specific deoxyribonuclease
• EC 3.1.21.4: type II site-specific deoxyribonuclease
• EC 3.1.21.5: type III site-specific deoxyribonuclease
• EC 3.1.21.6: CC-preferring endodeoxyribonuclease
• EC 3.1.21.7: deoxyribonuclease V

EC 3.1.22: Endodeoxyribonucleases Producing 3'-Phosphomonoesters[edit]

• EC 3.1.22.1: deoxyribonuclease II
• EC 3.1.22.2: Aspergillus deoxyribonuclease K1
• EC 3.1.22.3: now EC 3.1.21.7
• EC 3.1.22.4: crossover junction endodeoxyribonuclease
• EC 3.1.22.5: deoxyribonuclease X

EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5[edit]

EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases[edit]

• EC 3.1.25.1: deoxyribonuclease (pyrimidine dimer)
• EC 3.1.25.2: now EC 4.2.99.18

EC 3.1.26: Endoribonucleases Producing 5'-Phosphomonoesters[edit]

• EC 3.1.26.1: Physarum polycephalum ribonuclease
• EC 3.1.26.2: ribonuclease alpha
• EC 3.1.26.3: ribonuclease III
• EC 3.1.26.4: calf thymus ribonuclease H
• EC 3.1.26.5: ribonuclease P
• EC 3.1.26.6: ribonuclease IV
• EC 3.1.26.7: ribonuclease P4
• EC 3.1.26.8: ribonuclease M5
• EC 3.1.26.9: ribonuclease (poly-(U)-specific)
• EC 3.1.26.10: ribonuclease IX
• EC 3.1.26.11: ribonuclease Z
• EC 3.1.26.12: ribonuclease E
• EC 3.1.26.13: retroviral ribonuclease H
• EC 3.1.26.n1: now EC 3.1.26.12

EC 3.1.27: Endoribonucleases Producing 3'-Phosphomonoesters[edit]

• EC 3.1.27.1: ribonuclease T2
• EC 3.1.27.2: Bacillus subtilis ribonuclease
• EC 3.1.27.3: ribonuclease T1
• EC 3.1.27.4: ribonuclease U2
• EC 3.1.27.5: pancreatic ribonuclease
• EC 3.1.27.6: Enterobacter ribonuclease
• EC 3.1.27.7: ribonuclease F
• EC 3.1.27.8: ribonuclease V
• EC 3.1.27.9: tRNA-intron endonuclease
• EC 3.1.27.10: rRNA endonuclease

EC 3.1.30: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters[edit]

• EC 3.1.30.1: Aspergillus nuclease S1
• EC 3.1.30.2: Serratia marcescens nuclease

EC 3.1.31: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters[edit]

• EC 3.1.31.1: micrococcal nuclease

EC 3.2: Glycosylases[edit]

EC 3.2.1: Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds[edit]

• EC 3.2.1.1: α-amylase
• EC 3.2.1.2: β-amylase
• EC 3.2.1.3: glucan 1,4-a-glucosidase
• EC 3.2.1.4: cellulase
• EC 3.2.1.5: deleted
• EC 3.2.1.6: endo-1,3(4)-b-glucanase
• EC 3.2.1.7: inulinase
• EC 3.2.1.8: endo-1,4-β-xylanase
• EC 3.2.1.9: deleted
• EC 3.2.1.10: oligo-1,6-glucosidase
• EC 3.2.1.11: dextranase
• EC 3.2.1.12: deleted, included in EC 3.2.1.54
• EC 3.2.1.13: deleted, included in EC 3.2.1.54
• EC 3.2.1.14: chitinase
• EC 3.2.1.15: polygalacturonase
• EC 3.2.1.16: deleted
• EC 3.2.1.17: lysozyme
• EC 3.2.1.18: exo-a-sialidase
• EC 3.2.1.19: deleted
• EC 3.2.1.20: a-glucosidase
• EC 3.2.1.21: b-glucosidase
• EC 3.2.1.22: α-galactosidase
• EC 3.2.1.23: b-galactosidase
• EC 3.2.1.24: a-mannosidase
• EC 3.2.1.25: b-mannosidase
• EC 3.2.1.26: b-fructofuranosidase
• EC 3.2.1.27: deleted
• EC 3.2.1.28: α,α-trehalase
• EC 3.2.1.29: deleted, included in EC 3.2.1.52
• EC 3.2.1.30: deleted, included in EC 3.2.1.52
• EC 3.2.1.31: β-glucuronidase
• EC 3.2.1.32: xylan endo-1,3-b-xylosidase
• EC 3.2.1.33: amylo-1,6-glucosidase
• EC 3.2.1.34: deleted, included in EC 3.2.1.35
• EC 3.2.1.35: hyaluronoglucosaminidase
• EC 3.2.1.36: hyaluronoglucuronidase
• EC 3.2.1.37: xylan 1,4-b-xylosidase
• EC 3.2.1.38: b-D-fucosidase
• EC 3.2.1.39: glucan endo-1,3-b-D-glucosidase
• EC 3.2.1.40: a-L-rhamnosidase
• EC 3.2.1.41: pullulanase
• EC 3.2.1.42: GDP-glucosidase
• EC 3.2.1.43: b-L-rhamnosidase
• EC 3.2.1.44: fucoidanase
• EC 3.2.1.45: glucosylceramidase
• EC 3.2.1.46: galactosylceramidase
• EC 3.2.1.47: galactosylgalactosylglucosylceramidase
• EC 3.2.1.48: Sucrose alpha-glucosidase
• EC 3.2.1.49: a-N-acetylgalactosaminidase
• EC 3.2.1.50: α-N-acetylglucosaminidase
• EC 3.2.1.51: a-L-fucosidase
• EC 3.2.1.52: b-L-N-acetylhexosaminidase
• EC 3.2.1.53: b-N-acetylgalactosaminidase
• EC 3.2.1.54: cyclomaltodextrinase
• EC 3.2.1.55: a-N-arabinofuranosidase
• EC 3.2.1.56: glucuronosyl-disulfoglucosamine glucuronidase
• EC 3.2.1.57: isopullulanase
• EC 3.2.1.58: glucan 1,3-b-glucosidase
• EC 3.2.1.59: glucan endo-1,3-a-glucosidase
• EC 3.2.1.60: glucan 1,4-a-maltotetraohydrolase
• EC 3.2.1.61: mycodextranase
• EC 3.2.1.62: glycosylceramidase
• EC 3.2.1.63: 1,2-alpha-L-fucosidase
• EC 3.2.1.64: 2,6-b-fructan 6-levanbiohydrolase
• EC 3.2.1.65: levanase
• EC 3.2.1.66: quercitrinase
• EC 3.2.1.67: galacturan 1,4-a-galacturonidase
• EC 3.2.1.68: isoamylase
• EC 3.2.1.69: deleted, included in EC 3.2.1.41
• EC 3.2.1.70: glucan 1,6-a-glucosidase
• EC 3.2.1.71: glucan endo-1,2-b-glucosidase
• EC 3.2.1.72: xylan 1,3-b-xylosidase
• EC 3.2.1.73: licheninase
• EC 3.2.1.74: glucan 1,4-b-glucosidase
• EC 3.2.1.75: glucan endo-1,6-b-glucosidase
• EC 3.2.1.76: L-iduronidase
• EC 3.2.1.77: mannan 1,2-(1,3)-a-mannosidase
• EC 3.2.1.78: mannan endo-1,4-b-mannosidase
• EC 3.2.1.79: deleted, included in EC 3.2.1.55
• EC 3.2.1.80: fructan b-fructosidase
• EC 3.2.1.81: agarase
• EC 3.2.1.82: exo-poly-a-galacturonosidase
• EC 3.2.1.83: k-carrageenase
• EC 3.2.1.84: glucan 1,3-a-glucosidase
• EC 3.2.1.85: 6-phospho-b-galactosidase
• EC 3.2.1.86: 6-phospho-b-glucosidase
• EC 3.2.1.87: capsular-polysaccharide endo-1,3-a-galactosidase
• EC 3.2.1.88: b-L-arabinosidase
• EC 3.2.1.89: arabinogalactan endo-1,4-b-galactosidase
• EC 3.2.1.90: Deleted, not sufficiently characterised.
• EC 3.2.1.91: cellulose 1,4-b-cellobiosidase
• EC 3.2.1.92: peptidoglycan b-N-acetylmuramidase
• EC 3.2.1.93: a,a-phosphotrehalase
• EC 3.2.1.94: glucan 1,6-a-isomaltosidase
• EC 3.2.1.95: dextran 1,6-a-isomaltotriosidase
• EC 3.2.1.96: mannosyl-glycoprotein endo-b-N-acetylglucosaminidase
• EC 3.2.1.97: glycopeptide a-N-acetylgalactosaminidase
• EC 3.2.1.98: glucan 1,4-a-maltohexaosidase
• EC 3.2.1.99: arabinan endo-1,5-a-L-arabinosidase
• EC 3.2.1.100: mannan 1,4-mannobiosidase
• EC 3.2.1.101: mannan endo-1,6-a-mannosidase
• EC 3.2.1.102: blood-group-substance endo-1,4-b-galactosidase
• EC 3.2.1.103: keratan-sulfate endo-1,4-b-galactosidase
• EC 3.2.1.104: steryl-b-glucosidase
• EC 3.2.1.105: strictosidine b-glucosidase
• EC 3.2.1.106: mannosyl-oligosaccharide glucosidase
• EC 3.2.1.107: protein-glucosylgalactosylhydroxylysine glucosidase
• EC 3.2.1.108: lactase
• EC 3.2.1.109: endogalactosaminidase
• EC 3.2.1.110: mucinaminylserine mucinaminidase
• EC 3.2.1.111: 1,3-alpha-L-fucosidase
• EC 3.2.1.112: 2-deoxyglucosidase
• EC 3.2.1.113: mannosyl-oligosaccharide 1,2-a-mannosidase
• EC 3.2.1.114: mannosyl-oligosaccharide 1,3-1,6-a-mannosidase
• EC 3.2.1.115: branched-dextran exo-1,2-a-glucosidase
• EC 3.2.1.116: glucan 1,4-a-maltotriohydrolase
• EC 3.2.1.117: amygdalin b-glucosidase
• EC 3.2.1.118: prunasin b-glucosidase
• EC 3.2.1.119: vicianin b-glucosidase
• EC 3.2.1.120: oligoxyloglucan b-glycosidase
• EC 3.2.1.121: polymannuronate hydrolase
• EC 3.2.1.122: maltose-6'-phosphate glucosidase
• EC 3.2.1.123: endoglycosylceramidase
• EC 3.2.1.124: 3-deoxy-2-octulosonidase
• EC 3.2.1.125: raucaffricine b-glucosidase
• EC 3.2.1.126: coniferin b-glucosidase
• EC 3.2.1.127: 1,6-alpha-L-fucosidase
• EC 3.2.1.128: glycyrrhizinate beta-glucuronidase
• EC 3.2.1.129: endo-a-sialidase
• EC 3.2.1.130: glycoprotein endo-a-1,2-mannosidase
• EC 3.2.1.131: xylan a-1,2-glucuronosidase
• EC 3.2.1.132: chitosanase
• EC 3.2.1.133: glucan 1,4-a-maltohydrolase
• EC 3.2.1.134: difructose-anhydride synthase
• EC 3.2.1.135: neopullulanase
• EC 3.2.1.136: glucuronoarabinoxylan endo-1,4-b-xylanase
• EC 3.2.1.137: mannan exo-1,2-1,6-a-mannosidase
• EC 3.2.1.138: now EC 4.2.2.15
• EC 3.2.1.139: Alpha-glucuronidase
• EC 3.2.1.140: lacto-N-biosidase
• EC 3.2.1.141: 4-a-D-((1-4)-a-D-glucano)trehalose trehalohydrolase
• EC 3.2.1.142: limit dextrinase
• EC 3.2.1.143: poly(ADP-ribose) glycohydrolase
• EC 3.2.1.144: 3-deoxyoctulosonase
• EC 3.2.1.145: galactan 1,3-b-galactosidase
• EC 3.2.1.146: b-galactofuranosidase
• EC 3.2.1.147: thioglucosidase
• EC 3.2.1.148: now EC 4.4.1.21
• EC 3.2.1.149: b-primeverosidase
• EC 3.2.1.150: oligoxyloglucan reducing-end-specific cellobiohydrolase
• EC 3.2.1.151: xyloglucan-specific endo-b-1,4-glucanase
• EC 3.2.1.152: mannosylglycoprotein endo-b-mannosidase
• EC 3.2.1.153: fructan b-(2,1)-fructosidase
• EC 3.2.1.154: fructan b-(2,6)-fructosidase
• EC 3.2.1.155: xyloglucan-specific exo-beta-1,4-glucanase
• EC 3.2.1.156: oligosaccharide reducing-end xylanase
• EC 3.2.1.157: iota-carrageenase
• EC 3.2.1.158: alpha-agarase
• EC 3.2.1.159: alpha-neoagaro-oligosaccharide hydrolase
• EC 3.2.1.160: now EC 3.2.1.155
• EC 3.2.1.161: beta-apiosyl-beta-glucosidase
• EC 3.2.1.162: lambda-carrageenase
• EC 3.2.1.163: 1,6-alpha-D-mannosidase
• EC 3.2.1.164: galactan endo-1,6-beta-galactosidase
• EC 3.2.1.165: exo-1,4-beta-D-glucosaminidase
• EC 3.2.1.166: blood group B branched chain alpha-1,3-galactosidase
• EC 3.2.1.167: blood group B linear chain alpha-1,3-galactosidase
• EC 3.2.1.168: hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase
• EC 3.2.1.169: protein O-GlcNAcase
• EC 3.2.1.170: mannosylglycerate hydrolase
• EC 3.2.1.171: rhamnogalacturonan hydrolase
• EC 3.2.1.172: unsaturated rhamnogalacturonyl hydrolase
• EC 3.2.1.173: rhamnogalacturonan galacturonohydrolase
• EC 3.2.1.174: rhamnogalacturonan rhamnohydrolase
• EC 3.2.1.175: beta-D-glucopyranosyl abscisate beta-glucosidase
• EC 3.2.1.176: cellulose 1,4-beta-cellobiosidase (reducing end)
• EC 3.2.1.177: alpha-D-xyloside xylohydrolase
• EC 3.2.1.178: beta-porphyranase
• EC 3.2.1.179: gellan tetrasaccharide unsaturated glucuronyl hydrolase
• EC 3.2.1.180: unsaturated chondroitin disaccharide hydrolase
• EC 3.2.1.181: galactan endo-beta-1,3-galactanase
• EC 3.2.1.182: 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
• EC 3.2.1.183: UDP-N-acetylglucosamine 2-epimerase (hydrolysing)
• EC 3.2.1.184: UDP-N,N'-diacetylbacillosamine 2-epimerase (hydrolysing)

EC 3.2.2: Hydrolysing N-Glycosyl Compounds[edit]

• EC 3.2.2.1: purine nucleosidase
• EC 3.2.2.2: inosine nucleosidase
• EC 3.2.2.3: uridine nucleosidase
• EC 3.2.2.4: AMP nucleosidase
• EC 3.2.2.5: NAD+ nucleosidase
• EC 3.2.2.6: NAD(P)+ nucleosidase
• EC 3.2.2.7: adenosine nucleosidase
• EC 3.2.2.8: ribosylpyrimidine nucleosidase
• EC 3.2.2.9: adenosylhomocysteine nucleosidase
• EC 3.2.2.10: pyrimidine-5'-nucleotide nucleosidase
• EC 3.2.2.11: b-aspartyl-N-acetylglucosaminidase
• EC 3.2.2.12: inosinate nucleosidase
• EC 3.2.2.13: 1-methyladenosine nucleosidase
• EC 3.2.2.14: NMN nucleosidase
• EC 3.2.2.15: DNA-deoxyinosine glycosylase
• EC 3.2.2.16: methylthioadenosine nucleosidase
• EC 3.2.2.17: deoxyribodipyrimidine endonucleosidase
• EC 3.2.2.18: deleted, included in EC 3.5.1.52
• EC 3.2.2.19: ADP-ribosylarginine hydrolase
• EC 3.2.2.20: DNA-3-methyladenine glycosylase I
• EC 3.2.2.21: DNA-3-methyladenine glycosylase II
• EC 3.2.2.22: rRNA N-glycosylase
• EC 3.2.2.23: DNA-formamidopyrimidine glycosylase
• EC 3.2.2.24: ADP-ribosyl-(dinitrogen reductase) hydrolase
• EC 3.2.2.25: N-methyl nucleosidase
• EC 3.2.2.26: futalosine hydrolase
• EC 3.2.2.27: uracil-DNA glycosylase
• EC 3.2.2.28: double-stranded uracil-DNA glycosylase
• EC 3.2.2.29: thymine-DNA glycosylase

EC 3.2.3: Hydrolysing S-Glycosyl Compounds[edit]

• EC 3.2.3.1: now EC 3.2.1.147

EC 3.3: Acting on Ether Bonds[edit]

EC 3.3.1: Thioether and trialkylsulfonium hydrolases[edit]

• EC 3.3.1.1: adenosylhomocysteinase
• EC 3.3.1.2: adenosylmethionine hydrolase
• EC 3.3.1.3: now EC 3.2.1.148

EC 3.3.2: Ether Hydrolases[edit]

• EC 3.3.2.1: isochorismatase
• EC 3.3.2.2: alkenylglycerophosphocholine hydrolase
• EC 3.3.2.3: epoxide hydrolase
• EC 3.3.2.4: trans-epoxysuccinate hydrolase
• EC 3.3.2.5: alkenylglycerophosphoethanolamine hydrolase
• EC 3.3.2.6: leukotriene-A4 hydrolase
• EC 3.3.2.7: hepoxilin-epoxide hydrolase
• EC 3.3.2.8: limonene-1,2-epoxide hydrolase
• EC 3.3.2.9: microsomal epoxide hydrolase
• EC 3.3.2.10: soluble epoxide hydrolase
• EC 3.3.2.11: cholesterol-5,6-oxide hydrolase

EC 3.4: act on peptide bonds - Peptidase[edit]

EC 3.4.1 α-amino acyl peptide hydrolases (discontinued)[edit]

• EC 3.4.1.1: leucine aminopeptidase, now EC 3.4.11.1
• EC 3.4.1.2: alanine aminopeptidase, now EC 3.4.11.2
• EC 3.4.1.3: aminotripeptidase, now EC 3.4.11.4
• EC 3.4.1.4: proline iminopeptidase, now EC 3.4.11.5

EC 3.4.2 Peptidyl amino acid hydrolases (discontinued)[edit]

• EC 3.4.2.1: carboxypeptidase A, now EC 3.4.17.1
• EC 3.4.2.2: carboxypeptidase B, now EC 3.4.17.2
• EC 3.4.2.3: yeast carboxypeptidase, now EC 3.4.17.4

EC 3.4.3 Dipeptide Hydrolases (discontinued)[edit]

• EC 3.4.3.1: glycyl-glycine dipeptidase, now EC 3.4.13.18
• EC 3.4.3.2: glycyl-leucine dipeptidase, now EC 3.4.13.18
• EC 3.4.3.3: aminoacyl-histidine dipeptidase, carnosinase, now EC 3.4.13.3
• EC 3.4.3.4: aminoacyl-methylhistidine dipeptidase, anserinase, now EC 3.4.13.5
• EC 3.4.3.5: cysteinyl-glycine dipeptidase, now EC 3.4.13.6
• EC 3.4.3.6: iminodipeptidase, now EC 3.4.13.8
• EC 3.4.3.7: imidodipeptidase, prolidase, now EC 3.4.13.9

EC 3.4.4 Peptidyl Peptide Hydrolases (discontinued)[edit]

• EC 3.4.4.1: pepsin, now EC 3.4.23.1
• EC 3.4.4.2: pepsin B, now EC 3.4.23.2
• EC 3.4.4.3: rennin, now EC 3.4.23.4
• EC 3.4.4.4: trypsin, now EC 3.4.21.4
• EC 3.4.4.5: chymotrypsin, now EC 3.4.21.1
• EC 3.4.4.6: chymotrypsin B, now EC 3.4.21.1
• EC 3.4.4.7: pancreatopeptidase E, now EC 3.4.21.36 and EC 3.4.21.37
• EC 3.4.4.8: enteropeptidase, now EC 3.4.21.9
• EC 3.4.4.9: cathepsin C, now EC 3.4.14.1
• EC 3.4.4.10: papain now EC 3.4.22.2
• EC 3.4.4.11: chymopapain, now EC 3.4.22.6
• EC 3.4.4.12: ficin, now EC 3.4.22.3
• EC 3.4.4.13: thrombin, now EC 3.4.21.5
• EC 3.4.4.14: plasmin, now EC 3.4.21.7
• EC 3.4.4.15: renin, now EC 3.4.23.15
• EC 3.4.4.16: subtilopeptidase A, now EC 3.4.21.62 to EC 3.4.21.67
• EC 3.4.4.17: aspergillopeptidase A, now EC 3.4.23.20 to EC 3.4.23.30 3.4.23.30
• EC 3.4.4.18: streptococcus peptidase A, now EC 3.4.22.10
• EC 3.4.4.19: clostridiopeptidase A, now EC 3.4.24.3
• EC 3.4.4.20: clostridiopeptidase B, now EC 3.4.22.8
• EC 3.4.4.21: kallikrein, now EC 3.4.21.34
• EC 3.4.4.22: recommended Name never specified, now EC 3.4.23.3
• EC 3.4.4.23: recommended Name never specified, now EC 3.4.23.5
• EC 3.4.4.24: recommended Name never specified, now EC 3.4.22.32 and EC 3.4.22.33
• EC 3.4.4.25: keratinase deleted

EC 3.4.11 Aminopeptidases[edit]

• EC 3.4.11.1: leucyl aminopeptidase
• EC 3.4.11.2: membrane alanyl aminopeptidase
• EC 3.4.11.3: cystinyl aminopeptidase
• EC 3.4.11.4: tripeptide aminopeptidase
• EC 3.4.11.5: prolyl aminopeptidase
• EC 3.4.11.6: aminopeptidase B
• EC 3.4.11.7: glutamyl aminopeptidase
• EC 3.4.11.8: Transferred entry: pyroglutamyl aminopeptidase. Now EC 3.4.19.3, pyroglutamyl-peptidase I
• EC 3.4.11.9: X-Pro aminopeptidase
• EC 3.4.11.10: bacterial leucyl aminopeptidase
• EC 3.4.11.11: Deleted entry: aminopeptidase
• EC 3.4.11.12: Deleted entry: thermophilic aminopeptidase
• EC 3.4.11.13: Clostridial aminopeptidase
• EC 3.4.11.14: cytosol alanyl aminopeptidase
• EC 3.4.11.15: aminopeptidase Y
• EC 3.4.11.16: X-Trp aminopeptidase
• EC 3.4.11.17: tryptophanyl aminopeptidase
• EC 3.4.11.18: methionyl aminopeptidase
• EC 3.4.11.19: D-stereospecific aminopeptidase
• EC 3.4.11.20: aminopeptidase Ey
• EC 3.4.11.21: aspartyl aminopeptidase
• EC 3.4.11.22: aminopeptidase I
• EC 3.4.11.23: PepB aminopeptidase
• EC 3.4.11.24: aminopeptidase S
• EC 3.4.11.25: beta-peptidyl aminopeptidase
• EC 3.4.11.26: intermediate cleaving peptidase 55

EC 3.4.13 Dipeptidases[edit]

• EC 3.4.13.1: glycyl-glycine dipeptidase, now EC 3.4.13.18
• EC 3.4.13.2: glycyl-leucine dipeptidase, now EC 3.4.13.18
• EC 3.4.13.3: X-His dipeptidase
• EC 3.4.13.4: X-Arg dipeptidase
• EC 3.4.13.5: X-methyl-His dipeptidase
• EC 3.4.13.6: Transferred entry: Cys-Gly dipeptidase. Now EC 3.4.11.2, membrane alanyl aminopeptidase
• EC 3.4.13.7: Glu-Glu dipeptidase
• EC 3.4.13.8: Transferred entry: Pro-X dipeptidase. Now EC 3.4.13.18, cytosol nonspecific dipeptidase
• EC 3.4.13.9: X-Pro dipeptidase
• EC 3.4.13.10: Transferred entry: β-aspartyldipeptidase. Now EC 3.4.19.5, β-aspartyl-peptidase
• EC 3.4.13.11: Transferred entry: dipeptidase. Now EC 3.4.13.19, membrane dipeptidase
• EC 3.4.13.12: Met-X dipeptidase
• EC 3.4.13.13: Transferred entry: homocarnosinase. Now EC 3.4.13.3, X-His dipeptidase
• EC 3.4.13.14: Deleted entry: γ-glutamyldipeptidase
• EC 3.4.13.15: Transferred entry: N2-β-alanylarginine dipeptidase. Now EC 3.4.13.18, cytosol nonspecific dipeptidase
• EC 3.4.13.16: Deleted entry: aspartylphenylalanine dipeptidase
• EC 3.4.13.17: non-stereospecific dipeptidase
• EC 3.4.13.18: cytosol nonspecific dipeptidase
• EC 3.4.13.19: membrane dipeptidase
• EC 3.4.13.20: beta-Ala-His dipeptidase
• EC 3.4.13.21: dipeptidase E
• EC 3.4.13.22: D-Ala-D-Ala dipeptidase

EC 3.4.14 Dipeptidyl peptidases and tripeptidyl peptidases[edit]

• EC 3.4.14.1: dipeptidyl-peptidase I
• EC 3.4.14.2: dipeptidyl-peptidase II
• EC 3.4.14.3: Transferred entry: acylamino-acid-releasing enzyme. Now EC 3.4.19.1, acylaminoacyl-peptidase
• EC 3.4.14.4: dipeptidyl-peptidase III
• EC 3.4.14.5: dipeptidyl-peptidase IV
• EC 3.4.14.6: dipeptidyl-dipeptidase
• EC 3.4.14.7: Deleted entry: tetralysine endopeptidase
• EC 3.4.14.8: Transferred entry: tripeptidyl peptidase. Now EC 3.4.14.10, tripeptidyl-peptidase II
• EC 3.4.14.9: tripeptidyl-peptidase I
• EC 3.4.14.10: tripeptidyl-peptidase II
• EC 3.4.14.11: Xaa-Pro dipeptidyl-peptidase
• EC 3.4.14.12: Xaa-Xaa-Pro tripeptidyl-peptidase

EC 3.4.15 Peptidyl dipeptidases[edit]

• EC 3.4.15.1: peptidyl-dipeptidase A
• EC 3.4.15.2: Transferred entry: pepdidyl carboxyamidase. Now EC 3.4.19.2, peptidyl-glycinamidase
• EC 3.4.15.3: Transferred entry: dipeptidyl carboxypeptidase. Now EC 3.4.15.5, peptidyl-dipeptidase Dcp
• EC 3.4.15.4: Peptidyl-dipeptidase B
• EC 3.4.15.5: Peptidyl-dipeptidase Dcp
• EC 3.4.15.6: cyanophycinase

EC 3.4.16 Serine type carboxypeptidases[edit]

• EC 3.4.16.1: Transferred entry: serine carboxypeptidase. Now EC 3.4.16.6, carboxypeptidase D
• EC 3.4.16.2: lysosomal Pro-X carboxypeptidase
• EC 3.4.16.3: Transferred entry: tyrosine carboxypeptidase. Now included with EC 3.4.16.5, carboxypeptidase C
• EC 3.4.16.4: serine-type D-Ala-D-Ala carboxypeptidase
• EC 3.4.16.5: carboxypeptidase c
• EC 3.4.16.6: carboxypeptidase D

EC 3.4.17 Metallocarboxypeptidases[edit]

• EC 3.4.17.1: carboxypeptidase A
• EC 3.4.17.2: carboxypeptidase B
• EC 3.4.17.3: lysine carboxypeptidase
• EC 3.4.17.4: Gly-X carboxypeptidase
• EC 3.4.17.5: Deleted entry: aspartate carboxypeptidase
• EC 3.4.17.6: alanine carboxypeptidase
• EC 3.4.17.7: Transferred entry: acylmuramoyl-alanine carboxypeptidase. Now EC 3.5.1.28, N-acetylmuramoyl-L-alanine amidase
• EC 3.4.17.8: muramoylpentapeptide carboxypeptidase
• EC 3.4.17.9: Transferred entry: carboxypeptidase S. Now included with EC 3.4.17.4, Gly-Xaa carboxypeptidase
• EC 3.4.17.10: carboxypeptidase E
• EC 3.4.17.11: glutamate carboxypeptidase
• EC 3.4.17.12: carboxypeptidase M
• EC 3.4.17.13: Muramoyltetrapeptide carboxypeptidase
• EC 3.4.17.14: Zinc D-Ala-D-Ala carboxypeptidase
• EC 3.4.17.15: carboxypeptidase A2
• EC 3.4.17.16: Membrane Pro-X carboxypeptidase
• EC 3.4.17.17: tubulinyl-Tyr carboxypeptidase
• EC 3.4.17.18: carboxypeptidase T
• EC 3.4.17.19: Carboxypeptidase Taq
• EC 3.4.17.20: Carboxypeptidase u
• EC 3.4.17.21: Glutamate carboxypeptidase II
• EC 3.4.17.22: Metallocarboxypeptidase D
• EC 3.4.17.23: angiotensin-converting enzyme 2

EC 3.4.18 Cysteine type carboxypeptidases[edit]

• EC 3.4.18.1: cathepsin X

EC 3.4.19 Omega peptidases[edit]

• EC 3.4.19.1: acylaminoacyl-peptidase
• EC 3.4.19.2: peptidyl-glycinamidase
• EC 3.4.19.3: pyroglutamyl-peptidase I
• EC 3.4.19.4: Deleted entry: N-acetylmethionylpeptide peptidase
• EC 3.4.19.5: beta-aspartyl-peptidase
• EC 3.4.19.6: pyroglutamyl-peptidase II
• EC 3.4.19.7: N-Formylmethionyl-peptidase
• EC 3.4.19.8: Transferred entry: now EC 3.4.17.21, glutamate carboxypeptidase II
• EC 3.4.19.9: gamma-glutamyl hydrolase
• EC 3.4.19.10: Transferred entry: acylmuramoyl-Ala peptidase. Now EC 3.5.1.28, N-acetylmuramoyl-L-alanine amidase
• EC 3.4.19.11: gamma-D-Glutamyl-meso-diaminopimelate peptidase
• EC 3.4.19.12: ubiquitinyl hydrolase 1
• EC 3.4.19.13: glutathione hydrolase
• EC 3.4.19.14: leukotriene-C4 hydrolase

EC 3.4.21: Serine proteases[edit]

• EC 3.4.21.1: chymotrypsin
• EC 3.4.21.2: chymotrypsin C
• EC 3.4.21.3: metridin
• EC 3.4.21.4: trypsin
• EC 3.4.21.5: thrombin
• EC 3.4.21.6: coagulation factor Xa
• EC 3.4.21.7: plasmin
• EC 3.4.21.8: Transferred entry: kallikrein. Now EC 3.4.21.34 (plasma kallikrein) and EC 3.4.21.35 (tissue kallikrein)
• EC 3.4.21.9: enteropeptidase
• EC 3.4.21.10: acrosin
• EC 3.4.21.11: Transferred entry: elastase. Now EC 3.4.21.37, leukocyte elastase
• EC 3.4.21.12: alpha-lytic endopeptidase
• EC 3.4.21.13: Transferred entry: Phaseolus proteinase. Now EC 3.4.16.6, carboxypeptidase D
• EC 3.4.21.14: Transferred entry: now EC 3.4.21.67 endopeptidase So
• EC 3.4.21.15: Transferred entry: Aspergillus alkaline proteinase. Now EC 3.4.21.63, oryzin
• EC 3.4.21.16: Deleted entry: Alternaria serine proteinase
• EC 3.4.21.17: Deleted entry: Arthrobacter serine proteinase
• EC 3.4.21.18: Deleted entry: Tenebrio α-proteinase
• EC 3.4.21.19: glutamyl endopeptidase
• EC 3.4.21.20: cathepsin G
• EC 3.4.21.21: coagulation factor VIIa
• EC 3.4.21.22: coagulation factor IXa
• EC 3.4.21.23: Deleted entry: Vipera russelli proteinase
• EC 3.4.21.24: Deleted entry: red cell neutral endopeptidase
• EC 3.4.21.25: cucumisin
• EC 3.4.21.26: prolyl oligopeptidase
• EC 3.4.21.27: coagulation factor XIa
• EC 3.4.21.28: Transferred entry: Agkistrodon serine proteinase. Now EC 3.4.21.74, venombin A
• EC 3.4.21.29: Transferred entry: Bothrops atrox serine proteinase. Now EC 3.4.21.74, venombin A
• EC 3.4.21.30: Transferred entry: Crotalus adamanteus serine proteinase. Now EC 3.4.21.74, venombin A
• EC 3.4.21.31: Transferred entry: urokinase. Now EC 3.4.21.73, u-plasminogen activator
• EC 3.4.21.32: brachyurin
• EC 3.4.21.33: Deleted entry: Entomophthora collagenolytic proteinase
• EC 3.4.21.34: plasma kallikrein
• EC 3.4.21.35: renal tissue kallikrein
• EC 3.4.21.36: pancreatic elastase
• EC 3.4.21.37: leukocyte elastase
• EC 3.4.21.38: coagulation factor XIIa
• EC 3.4.21.39: chymase
• EC 3.4.21.40: Deleted entry: submandibular proteinase A
• EC 3.4.21.41: complement subcomponent C1r
• EC 3.4.21.42: complement subcomponent C1s
• EC 3.4.21.43: classical-complement-pathway C3/C5 convertase
• EC 3.4.21.44: Transferred entry: complement component C5 convertase. Now EC 3.4.21.43, classical-complement-pathway C3/C5 convertase
• EC 3.4.21.45: complement factor I
• EC 3.4.21.46: complement factor D
• EC 3.4.21.47: alternative-complement-pathway C3/C5 convertase
• EC 3.4.21.48: cerevisin
• EC 3.4.21.49: hypodermin C
• EC 3.4.21.50: lysyl endopeptidase
• EC 3.4.21.51: Deleted entry: Leukocyte-membrane neutral endopeptidase
• EC 3.4.21.52: Deleted entry: Cathepsin R
• EC 3.4.21.53: Endopeptidase La
• EC 3.4.21.54: gamma-Renin
• EC 3.4.21.55: Venombin AB
• EC 3.4.21.56: Deleted entry: euphorbain. Now considered EC 3.4.21.25, cucumisin
• EC 3.4.21.57: Leucyl endopeptidase
• EC 3.4.21.58: Deleted entry: prohormone serine proteinase
• EC 3.4.21.59: Tryptase
• EC 3.4.21.60: Scutelarin
• EC 3.4.21.61: Kexin
• EC 3.4.21.62: Subtilisin
• EC 3.4.21.63: Oryzin
• EC 3.4.21.64: Endopeptidase K
• EC 3.4.21.65: Thermomycolin
• EC 3.4.21.66: Thermitase
• EC 3.4.21.67: Endopeptidase So
• EC 3.4.21.68: t-Plasminogen activator
• EC 3.4.21.69: Protein C (activated)
• EC 3.4.21.70: Pancreatic endopeptidase E
• EC 3.4.21.71: Pancreatic elastase II
• EC 3.4.21.72: IgA-specific serine endopeptidase
• EC 3.4.21.73: u-Plasminogen activator
• EC 3.4.21.74: Venombin A
• EC 3.4.21.75: Furin
• EC 3.4.21.76: Myeloblastin
• EC 3.4.21.77: semenogelase
• EC 3.4.21.78: Granzyme A
• EC 3.4.21.79: Granzyme B
• EC 3.4.21.80: Streptogrisin A
• EC 3.4.21.81: Streptogrisin B
• EC 3.4.21.82: Glutamyl endopeptidase II
• EC 3.4.21.83: Oligopeptidase B
• EC 3.4.21.84: Limulus clotting factor C
• EC 3.4.21.85: Limulus clotting factor B
• EC 3.4.21.86: Limulus clotting enzyme
• EC 3.4.21.87: Transferred entry: omptin. Now EC 3.4.23.49, omptin. The enzyme is not a serine protease, as thought previously, but an aspartate protease
• EC 3.4.21.88: Repressor LexA
• EC 3.4.21.89: Signal peptidase I
• EC 3.4.21.90: Togavirin
• EC 3.4.21.91: Flavivirin
• EC 3.4.21.92: Endopeptidase Clp
• EC 3.4.21.93: Proprotein convertase 1
• EC 3.4.21.94: Proprotein convertase 2
• EC 3.4.21.95: Snake venom factor V activator
• EC 3.4.21.96: Lactocepin
• EC 3.4.21.97: assemblin
• EC 3.4.21.98: hepacivirin
• EC 3.4.21.99: spermosin
• EC 3.4.21.100: pseudomonalisin
• EC 3.4.21.101: xanthomonalisin
• EC 3.4.21.102: C-terminal processing peptidase
• EC 3.4.21.103: physarolisin
• EC 3.4.21.104: mannan-binding lectin-associated serine protease-2
• EC 3.4.21.105: rhomboid protease
• EC 3.4.21.106: hepsin
• EC 3.4.21.107: peptidase Do
• EC 3.4.21.108: HtrA2 peptidase
• EC 3.4.21.109: matriptase
• EC 3.4.21.110: C5a peptidase
• EC 3.4.21.111: aqualysin 1
• EC 3.4.21.112: site-1 protease
• EC 3.4.21.113: pestivirus NS3 polyprotein peptidase
• EC 3.4.21.114: equine arterivirus serine peptidase
• EC 3.4.21.115: infectious pancreatic necrosis birnavirus Vp4 peptidase
• EC 3.4.21.116: SpoIVB peptidase
• EC 3.4.21.117: stratum corneum chymotryptic enzyme
• EC 3.4.21.118: kallikrein 8
• EC 3.4.21.119: kallikrein 13
• EC 3.4.21.120: oviductin

EC 3.4.22 Cysteine proteases[edit]

• EC 3.4.22.1: cathepsin B
• EC 3.4.22.2: papain
• EC 3.4.22.3: ficain
• EC 3.4.22.4: bromelain (stem). Now EC 3.4.22.32 (stem bromelain) and EC 3.4.22.33 (fruit bromelain)
• EC 3.4.22.5: bromelain (juice). Now EC 3.4.22.32 (stem bromelain) and EC 3.4.22.33 (fruit bromelain)
• EC 3.4.22.6: chymopapain
• EC 3.4.22.7: asclepain
• EC 3.4.22.8: clostripain
• EC 3.4.22.9: yeast proteinase B. Now EC 3.4.21.48, cerevisin
• EC 3.4.22.10: streptopain
• EC 3.4.22.11: insulinase. Now EC 3.4.24.56, insulysin
• EC 3.4.22.12: gamma-glutamyl hydrolase. Now EC 3.4.19.9, γ-glutamyl hydrolase
• EC 3.4.22.13: Deleted entry: staphylococcal cysteine proteinase
• EC 3.4.22.14: actinidain
• EC 3.4.22.15: cathepsin L
• EC 3.4.22.16: cathepsin H
• EC 3.4.22.17: Transferred entry: calpain. Now EC 3.4.22.53, calpain-2
• EC 3.4.22.18: Transferred entry: prolyl endopeptidase (thiol-dependent). Now EC 3.4.21.26, prolyl oligopeptidase
• EC 3.4.22.19: ransferred entry: endo-oligopeptidase. Now EC 3.4.24.15, thimet oligopeptidase
• EC 3.4.22.20: Deleted entry: dinorphin-converting enzyme
• EC 3.4.22.21: Transferred entry: yeast cysteine proteinase E. Now EC 3.4.25.1, proteasome endopeptidase complex
• EC 3.4.22.22: Transferred entry: yeast cysteine proteinase D. Now EC 3.4.24.37, saccharolysin
• EC 3.4.22.23: Transferred entry: yeast cysteine proteinase F. Now EC 3.4.21.61, kexin
• EC 3.4.22.24: Cathepsin T
• EC 3.4.22.25: Glycyl endopeptidase
• EC 3.4.22.26: Cancer procoagulant
• EC 3.4.22.27: cathepsin S
• EC 3.4.22.28: picornain 3C
• EC 3.4.22.29: picornain 2A
• EC 3.4.22.30: Caricain
• EC 3.4.22.31: Ananain
• EC 3.4.22.32: Stem bromelain
• EC 3.4.22.33: Fruit bromelain
• EC 3.4.22.34: Legumain
• EC 3.4.22.35: Histolysain
• EC 3.4.22.36: caspase-1
• EC 3.4.22.37: Gingipain R
• EC 3.4.22.38: Cathepsin K
• EC 3.4.22.39: adenain
• EC 3.4.22.40: bleomycin hydrolase
• EC 3.4.22.41: cathepsin F
• EC 3.4.22.42: cathepsin O
• EC 3.4.22.43: cathepsin V
• EC 3.4.22.44: nuclear-inclusion-a endopeptidase
• EC 3.4.22.45: helper-component proteinase
• EC 3.4.22.46: L-peptidase
• EC 3.4.22.47: gingipain K
• EC 3.4.22.48: staphopain
• EC 3.4.22.49: separase
• EC 3.4.22.50: V-cath endopeptidase
• EC 3.4.22.51: cruzipain
• EC 3.4.22.52: calpain-1
• EC 3.4.22.53: calpain-2
• EC 3.4.22.54: calpain-3
• EC 3.4.22.55: caspase-2
• EC 3.4.22.56: caspase-3
• EC 3.4.22.57: caspase-4
• EC 3.4.22.58: caspase-5
• EC 3.4.22.59: caspase-6
• EC 3.4.22.60: caspase-7
• EC 3.4.22.61: caspase-8
• EC 3.4.22.62: caspase-9
• EC 3.4.22.63: caspase-10
• EC 3.4.22.64: caspase-11
• EC 3.4.22.65: peptidase 1 (mite)
• EC 3.4.22.66: calicivirin
• EC 3.4.22.67: zingipain
• EC 3.4.22.68: Ulp1 peptidase
• EC 3.4.22.69: SARS coronavirus main proteinase
• EC 3.4.22.70: sortase A
• EC 3.4.22.71: sortase B

EC 3.4.23 Aspartic endopeptidases[edit]

• EC 3.4.23.1: pepsin A
• EC 3.4.23.2: pepsin B
• EC 3.4.23.3: gastricsin
• EC 3.4.23.4: chymosin
• EC 3.4.23.5: cathepsin D
• EC 3.4.23.6: now EC 3.4.23.30 pycnoporopepsin
• EC 3.4.23.7: Penicillium janthinellum acid proteinase. Now EC 3.4.23.20, penicillopepsin
• EC 3.4.23.8: yeast proteinase A. Now EC 3.4.23.25, saccharopepsin
• EC 3.4.23.9: Rhizopus acid proteinase. Now EC 3.4.23.21, rhizopuspepsin
• EC 3.4.23.10: Endothia acid proteinase. Now EC 3.4.23.22, endothiapepsin
• EC 3.4.23.11: Deleted entry: thyroid aspartic proteinase
• EC 3.4.23.12: nepenthesin
• EC 3.4.23.13: Deleted entry: Lotus aspartic proteinase
• EC 3.4.23.14: Deleted entry: sorghum aspartic proteinase
• EC 3.4.23.15: renin
• EC 3.4.23.16: HIV-1 retropepsin
• EC 3.4.23.17: Pro-opiomelanocortin converting enzyme
• EC 3.4.23.18: Aspergillopepsin I
• EC 3.4.23.19: Aspergillopepsin II
• EC 3.4.23.20: Penicillopepsin
• EC 3.4.23.21: Rhizopuspepsin
• EC 3.4.23.22: Endothiapepsin
• EC 3.4.23.23: Mucorpepsin
• EC 3.4.23.24: Candidapepsin
• EC 3.4.23.25: Saccharopepsin
• EC 3.4.23.26: Rhodotorulapepsin
• EC 3.4.23.27: physaropepsin. Now EC 3.4.21.103, physarolisin
• EC 3.4.23.28: Acrocylindropepsin
• EC 3.4.23.29: Polyporopepsin
• EC 3.4.23.30: Pycnoporopepsin
• EC 3.4.23.31: Scytalidopepsin A
• EC 3.4.23.32: Scytalidopepsin B
• EC 3.4.23.33: xanthomonapepsin. Now EC 3.4.21.101, xanthomonalisin
• EC 3.4.23.34: Cathepsin e
• EC 3.4.23.35: Barrierpepsin
• EC 3.4.23.36: Signal peptidase II
• EC 3.4.23.37: pseudomonapepsin. Now EC 3.4.21.100, pseudomonalisin
• EC 3.4.23.38: plasmepsin I
• EC 3.4.23.39: plasmepsin II
• EC 3.4.23.40: Phytepsin
• EC 3.4.23.41: yapsin 1
• EC 3.4.23.42: thermopsin
• EC 3.4.23.43: prepilin peptidase
• EC 3.4.23.44: nodavirus endopeptidase
• EC 3.4.23.45: memapsin 1
• EC 3.4.23.46: memapsin 2
• EC 3.4.23.47: HIV-2 retropepsin
• EC 3.4.23.48: plasminogen activator Pla
• EC 3.4.23.49: omptin
• EC 3.4.23.50: human endogenous retrovirus K endopeptidase
• EC 3.4.23.51: HycI peptidase
• EC 3.4.23.52: preflagellin peptidase

EC 3.4.24: Metallopeptidases[edit]

• EC 3.4.24.1: atrolysin A
• EC 3.4.24.2: Deleted entry: Sepia proteinase
• EC 3.4.24.3: microbial collagenase
• EC 3.4.24.4: now EC 3.4.24.40 serralysin
• EC 3.4.24.5: Deleted entry: lens neutral proteinase. Now included with EC 3.4.22.53 (calpain-2) and EC 3.4.25.1 (proteasome endopeptidase complex)
• EC 3.4.24.6: leucolysin
• EC 3.4.24.7: interstitial collagenase
• EC 3.4.24.8: Transferred entry: Achromobacter iophagus collagenase. Now EC 3.4.24.3, microbial collagenase
• EC 3.4.24.9: Deleted entry: Trichophyton schoenleinii collagenase
• EC 3.4.24.10: Deleted entry: Trichophyton mentagrophytes keratinase
• EC 3.4.24.11: neprilysin
• EC 3.4.24.12: envelysin
• EC 3.4.24.13: IgA-specific metalloendopeptidase
• EC 3.4.24.14: procollagen N-endopeptidase
• EC 3.4.24.15: thimet oligopeptidase
• EC 3.4.24.16: neurolysin
• EC 3.4.24.17: stromelysin 1
• EC 3.4.24.18: meprin A
• EC 3.4.24.19: procollagen C-endopeptidase
• EC 3.4.24.20: peptidyl-Lys metalloendopeptidase
• EC 3.4.24.21: astacin
• EC 3.4.24.22: stromelysin 2
• EC 3.4.24.23: matrilysin
• EC 3.4.24.24: gelatinase a
• EC 3.4.24.25: vibriolysin
• EC 3.4.24.26: pseudolysin
• EC 3.4.24.27: thermolysin
• EC 3.4.24.28: bacillolysin
• EC 3.4.24.29: aureolysin
• EC 3.4.24.30: coccolysin
• EC 3.4.24.31: mycolysin
• EC 3.4.24.32: beta-Lytic metalloendopeptidase
• EC 3.4.24.33: peptidyl-Asp metalloendopeptidase
• EC 3.4.24.34: neutrophil collagenase
• EC 3.4.24.35: gelatinase B
• EC 3.4.24.36: leishmanolysin
• EC 3.4.24.37: saccharolysin
• EC 3.4.24.38: gametolysin
• EC 3.4.24.39: deuterolysin
• EC 3.4.24.40: serralysin
• EC 3.4.24.41: atrolysin B
• EC 3.4.24.42: atrolysin C
• EC 3.4.24.43: atroxase
• EC 3.4.24.44: atrolysin E
• EC 3.4.24.45: atrolysin F
• EC 3.4.24.46: adamalysin
• EC 3.4.24.47: horrilysin
• EC 3.4.24.48: ruberlysin
• EC 3.4.24.49: bothropasin
• EC 3.4.24.50: bothrolysin
• EC 3.4.24.51: ophiolysin
• EC 3.4.24.52: trimerelysin I
• EC 3.4.24.53: trimerelysin II
• EC 3.4.24.54: mucrolysin
• EC 3.4.24.55: pitrilysin
• EC 3.4.24.56: insulysin
• EC 3.4.24.57: O-sialoglycoprotein endopeptidase
• EC 3.4.24.58: russellysin
• EC 3.4.24.59: mitochondrial intermediate peptidase
• EC 3.4.24.60: dactylysin
• EC 3.4.24.61: nardilysin
• EC 3.4.24.62: magnolysin
• EC 3.4.24.63: meprin B
• EC 3.4.24.64: mitochondrial processing peptidase
• EC 3.4.24.65: macrophage elastase
• EC 3.4.24.66: choriolysin L
• EC 3.4.24.67: choriolysin H
• EC 3.4.24.68: tentoxilysin
• EC 3.4.24.69: bontoxilysin
• EC 3.4.24.70: oligopeptidase A
• EC 3.4.24.71: endothelin-converting enzyme 1
• EC 3.4.24.72: fibrolase
• EC 3.4.24.73: jararhagin
• EC 3.4.24.74: fragilysin
• EC 3.4.24.75: lysostaphin
• EC 3.4.24.76: flavastacin
• EC 3.4.24.77: snapalysin
• EC 3.4.24.78: gpr endopeptidase
• EC 3.4.24.79: pappalysin-1
• EC 3.4.24.80: membrane-type matrix metalloproteinase-1
• EC 3.4.24.81: ADAM10 endopeptidase
• EC 3.4.24.82: ADAMTS-4 endopeptidase
• EC 3.4.24.83: anthrax lethal factor endopeptidase
• EC 3.4.24.84: Ste24 endopeptidase
• EC 3.4.24.85: S2P endopeptidase
• EC 3.4.24.86: ADAM 17 endopeptidase
• EC 3.4.24.87: ADAMTS13 endopeptidase

EC 3.4.25 Threonine endopeptidases[edit]

• EC 3.4.25.1: proteasome endopeptidase complex
• EC 3.4.25.2: HslU—HslV peptidase

EC 3.4.99 Endopeptidases of unknown catalytic mechanism[edit]

• EC 3.4.99.1: acrocylindricum proteinase. Now EC 3.4.23.28, acrocylindropepsin
• EC 3.4.99.2: Deleted entry: agavain
• EC 3.4.99.3: Deleted entry: angiotensinase
• EC 3.4.99.4: Transferred entry: aspartylendopeptidase. Now EC 3.4.23.12, nepenthesin
• EC 3.4.99.5: Transferred entry: Clostridium histolyticum collagenase 2. Now EC 3.4.24.3, microbial collagenase
• EC 3.4.99.6: Transferred entry: crayfish low-molecular-weight proteinase. Now EC 3.4.24.21, astacin
• EC 3.4.99.7: Deleted entry: euphorbain
• EC 3.4.99.8: Deleted entry: Gliocladium proteinase
• EC 3.4.99.9: Deleted entry: hurain. Now considered EC 3.4.21.25, cucumisin
• EC 3.4.99.10: Transferred entry: insulinase. Now EC 3.4.24.56, insulysin
• EC 3.4.99.11: Deleted entry: Streptomyces alkalophilic keratinase
• EC 3.4.99.12: Deleted entry: Trichophyton mentagrophytes keratinase
• EC 3.4.99.13: Transferred entry: β-lytic proteinase (Mycobacterium sorangium). Now EC 3.4.24.32, β-lytic metalloendopeptidase
• EC 3.4.99.14: Deleted entry: mexicanain
• EC 3.4.99.15: Deleted entry: Paecilomyces proteinase
• EC 3.4.99.16: Deleted entry: Penicillium notatum extracellular proteinase
• EC 3.4.99.17: Deleted entry: peptidoglycan endopeptidase
• EC 3.4.99.18: Deleted entry: pinguinain
• EC 3.4.99.19: Transferred entry: renin. Now EC 3.4.23.15, renin
• EC 3.4.99.20: Deleted entry: scopulariopsis proteinase
• EC 3.4.99.21: Deleted entry: solanain. Now considered EC 3.4.21.25, cucumisin
• EC 3.4.99.22: Transferred entry: staphylokinase. Now EC 3.4.24.29, aureolysin
• EC 3.4.99.23: Deleted entry: tabernamontanain. Now considered EC 3.4.21.25, cucumisin
• EC 3.4.99.24: Deleted entry: Tenebrio α-proteinase
• EC 3.4.99.25: Transferred entry: trametes acid proteinase. Now EC 3.4.23.21, rhizopuspepsin
• EC 3.4.99.26: Transferred entry: urokinase. Now EC 3.4.21.68, t-plasminogen activator
• EC 3.4.99.27: Deleted entry: Echis carinatus prothrombin-activating proteinase
• EC 3.4.99.28: Transferred entry: Oxyuranus scutellatus prothrombin-activating proteinase. Now EC 3.4.21.60, scutelarin
• EC 3.4.99.29: Deleted entry: Myxobacter AL-1 proteinase I
• EC 3.4.99.30: Transferred entry: Myxobacter AL-1 proteinase II. Now EC 3.4.24.20, peptidyl-Lys metalloendopeptidase
• EC 3.4.99.31: Transferred entry: tissue endopeptidase degrading collagenase synthetic substrate. Now EC 3.4.24.15, thimet oligopeptidase
• EC 3.4.99.32: Transferred entry: Armillaria mellea neutral proteinase. Now EC 3.4.24.20, peptidyl-Lys metalloendopeptidase
• EC 3.4.99.33: Deleted entry: cathepsin R
• EC 3.4.99.34: Deleted entry: mytilidase
• EC 3.4.99.35: Transferred entry: premurein-leader peptidase. Now EC 3.4.23.36, signal peptidase II
• EC 3.4.99.36: Transferred entry: leader peptidase. Now EC 3.4.21.89, signal peptidase I
• EC 3.4.99.37: Deleted entry: RecA peptidase
• EC 3.4.99.38: Transferred entry: pro-opiomelanotropin-converting proteinase. Now EC 3.4.23.17, pro-opiomelanocortin converting enzyme
• EC 3.4.99.39: Deleted entry: pseudomurein endopeptidase
• EC 3.4.99.40: Deleted entry: pro-gonadoliberin proteinase
• EC 3.4.99.41: Transferred entry: mitochondrial processing peptidase. Now EC 3.4.24.64, mitochondrial processing peptidase
• EC 3.4.99.42: Deleted entry: leucyllysine endopeptidase
• EC 3.4.99.43: Transferred entry: thermopsin. Now EC 3.4.23.42, thermopsin
• EC 3.4.99.44: Transferred entry: pitrilysin. Now EC 3.4.24.55, pitrilysin
• EC 3.4.99.45: Transferred entry: insulinase. Now EC 3.4.24.56, insulysin
• EC 3.4.99.46: Transferred entry: multicatalytic endopeptidase complex. Now EC 3.4.25.1, proteasome endopeptidase complex

EC 3.5: act on carbon-nitrogen bonds, other than peptide bonds[edit]

3.5.1: linear amides[edit]

• EC 3.5.1.1: asparaginase
• EC 3.5.1.2: glutaminase
• EC 3.5.1.3: omega-amidase
• EC 3.5.1.4: amidase
• EC 3.5.1.5: urease
• EC 3.5.1.6: beta-ureidopropionase
• EC 3.5.1.7: ureidosuccinase
• EC 3.5.1.8: formylaspartate deformylase
• EC 3.5.1.9: arylformamidase
• EC 3.5.1.10: formyltetrahydrofolate deformylase
• EC 3.5.1.11: penicillin amidase
• EC 3.5.1.12: biotinidase
• EC 3.5.1.13: aryl-acylamidase
• EC 3.5.1.14: aminoacylase
• EC 3.5.1.15: aspartoacylase
• EC 3.5.1.16: acetylornithine deacetylase
• EC 3.5.1.17: acyl-lysine deacylase
• EC 3.5.1.18: succinyl-diaminopimelate desuccinylase
• EC 3.5.1.19: nicotinamidase
• EC 3.5.1.20: citrullinase
• EC 3.5.1.21: N-acetyl-beta-alanine deacetylase
• EC 3.5.1.22: pantothenase
• EC 3.5.1.23: ceramidase
• EC 3.5.1.24: choloylglycine hydrolase
• EC 3.5.1.25: N-acetylglucosamine-6-phosphate deacetylase
• EC 3.5.1.26: N4-(beta-N-acetylglucosaminyl)-L-asparaginase
• EC 3.5.1.27: N-formylmethionylaminoacyl-tRNA deformylase
• EC 3.5.1.28: N-acetylmuramoyl-L-alanine amidase
• EC 3.5.1.29: 2-(acetamidomethylene)succinate hydrolase
• EC 3.5.1.30: 5-aminopentanamidase
• EC 3.5.1.31: formylmethionine deformylase
• EC 3.5.1.32: hippurate hydrolase
• EC 3.5.1.33: N-acetylglucosamine deacetylase
• EC 3.5.1.34: acetylhistidine deacetylase
• EC 3.5.1.35: D-glutaminase
• EC 3.5.1.36: N-methyl-2-oxoglutaramate hydrolase
• EC 3.5.1.37: Deleted entry: 4-L-aspartylglycosylamine amidohydrolase. Identical with EC 3.5.1.26 N4-(β-N-acetylglucosaminyl)-L-asparaginase
• EC 3.5.1.38: glutamin-(asparagin-)ase
• EC 3.5.1.39: alkylamidase
• EC 3.5.1.40: acylagmatine amidase
• EC 3.5.1.41: chitin deacetylase
• EC 3.5.1.42: nicotinamide-nucleotide amidase
• EC 3.5.1.43: peptidyl-glutaminase
• EC 3.5.1.44: protein-glutamine glutaminase
• EC 3.5.1.45: Deleted entry: urease (ATP-hydrolysing). Now listed only as EC 6.3.4.6 urea carboxylase
• EC 3.5.1.46: 6-aminohexanoate-dimer hydrolase
• EC 3.5.1.47: N-acetyldiaminopimelate deacetylase
• EC 3.5.1.48: acetylspermidine deacetylase
• EC 3.5.1.49: formamidase
• EC 3.5.1.50: pentanamidase
• EC 3.5.1.51: 4-acetamidobutyryl-CoA deacetylase
• EC 3.5.1.52: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
• EC 3.5.1.53: N-carbamoylputrescine amidase
• EC 3.5.1.54: allophanate hydrolase
• EC 3.5.1.55: long-chain-fatty-acyl-glutamate deacylase
• EC 3.5.1.56: N,N-dimethylformamidase
• EC 3.5.1.57: tryptophanamidase
• EC 3.5.1.58: N-benzyloxycarbonylglycine hydrolase
• EC 3.5.1.59: N-carbamoylsarcosine amidase
• EC 3.5.1.60: N-(long-chain-acyl)ethanolamine deacylase
• EC 3.5.1.61: mimosinase
• EC 3.5.1.62: acetylputrescine deacetylase
• EC 3.5.1.63: 4-acetamidobutyrate deacetylase
• EC 3.5.1.64: Nalpha-benzyloxycarbonylleucine hydrolase
• EC 3.5.1.65: theanine hydrolase
• EC 3.5.1.66: 2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase
• EC 3.5.1.67: 4-methyleneglutaminase
• EC 3.5.1.68: N-formylglutamate deformylase
• EC 3.5.1.69: glycosphingolipid deacylase
• EC 3.5.1.70: aculeacin-A deacylase
• EC 3.5.1.71: N-feruloylglycine deacylase
• EC 3.5.1.72: D-benzoylarginine-4-nitroanilide amidase
• EC 3.5.1.73: carnitinamidase
• EC 3.5.1.74: chenodeoxycholoyltaurine hydrolase
• EC 3.5.1.75: urethanase
• EC 3.5.1.76: Arylalkyl acylamidase
• EC 3.5.1.77: N-carbamoyl-D-amino acid hydrolase
• EC 3.5.1.78: Glutathionylspermidine amidase
• EC 3.5.1.79: Phthalyl amidase
• EC 3.5.1.80: Deleted entry: N-acetylgalactosamine-6-phosphate deacetylase. Identical to EC 3.5.1.25, N-acetylglucosamine-6-phosphate deacetylase
• EC 3.5.1.81: N-acyl-D-amino-acid deacylase
• EC 3.5.1.82: N-acyl-D-glutamate deacylase
• EC 3.5.1.83: N-acyl-D-aspartate deacylase
• EC 3.5.1.84: biuret amidohydrolase
• EC 3.5.1.85: (S)-N-acetyl-1-phenylethylamine hydrolase
• EC 3.5.1.86: mandelamide amidase
• EC 3.5.1.87: N-carbamoyl-L-amino-acid hydrolase
• EC 3.5.1.88: peptide deformylase
• EC 3.5.1.89: N-acetylglucosaminylphosphatidylinositol deacetylase
• EC 3.5.1.90: adenosylcobinamide hydrolase
• EC 3.5.1.91: N-substituted formamide deformylase
• EC 3.5.1.92: pantetheine hydrolase
• EC 3.5.1.93: glutaryl-7-aminocephalosporanic-acid acylase
• EC 3.5.1.94: gamma-glutamyl-gamma-aminobutyrate hydrolase
• EC 3.5.1.95: N-malonylurea hydrolase
• EC 3.5.1.96: succinylglutamate desuccinylase
• EC 3.5.1.97: acyl-homoserine-lactone acylase
• EC 3.5.1.98: histone deacetylase
• EC 3.5.1.99: fatty acid amide hydrolase
• EC 3.5.1.100: (R)-amidase
• EC 3.5.1.101: L-proline amide hydrolase
• EC 3.5.1.102: 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase
• EC 3.5.1.103: N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase
• EC 3.5.1.104: peptidoglycan-N-acetylglucosamine deacetylase
• EC 3.5.1.105: chitin disaccharide deacetylase
• EC 3.5.1.106: N-formylmaleamate deformylase
• EC 3.5.1.107: maleamate amidohydrolase
• EC 3.5.1.108: UDP-3-O-acyl-N-acetylglucosamine deacetylase
• EC 3.5.1.109: sphingomyelin deacylase
• EC 3.5.1.110: peroxyureidoacrylate/ureidoacrylate amidohydrolase
• EC 3.5.1.111: 2-oxoglutaramate amidase
• EC 3.5.1.112: 2'-N-acetylparomamine deacetylase
• EC 3.5.1.113: 2-acetyl-6-hydroxyneomycin C deacetylase

3.5.2: cyclic amides[edit]

• EC 3.5.2.1: barbiturase
• EC 3.5.2.2: dihydropyrimidinase
• EC 3.5.2.3: dihydroorotase
• EC 3.5.2.4: carboxymethylhydantoinase
• EC 3.5.2.5: allantoinase
• EC 3.5.2.6: beta-lactamase
• EC 3.5.2.7: imidazolonepropionase
• EC 3.5.2.8: Deleted entry: cephalosporinase. Now included with EC 3.5.2.6 β-lactamase
• EC 3.5.2.9: 5-oxoprolinase (ATP-hydrolysing)
• EC 3.5.2.10: creatininase
• EC 3.5.2.11: L-lysine-lactamase
• EC 3.5.2.12: 6-aminohexanoate-cyclic-dimer hydrolase
• EC 3.5.2.13: 2,5-dioxopiperazine hydrolase
• EC 3.5.2.14: N-methylhydantoinase (ATP-hydrolysing)
• EC 3.5.2.15: cyanuric acid amidohydrolase
• EC 3.5.2.16: maleimide hydrolase
• EC 3.5.2.17: hydroxyisourate hydrolase
• EC 3.5.2.18: enamidase
• EC 3.5.2.19: streptothricin hydrolase

3.5.3: linear amidines[edit]

• EC 3.5.3.1: arginase
• EC 3.5.3.2: guanidinoacetase
• EC 3.5.3.3: creatinase
• EC 3.5.3.4: allantoicase
• EC 3.5.3.5: formimidoylaspartate deiminase
• EC 3.5.3.6: arginine deiminase
• EC 3.5.3.7: guanidinobutyrase
• EC 3.5.3.8: formimidoylglutamase
• EC 3.5.3.9: allantoate deiminase
• EC 3.5.3.10: D-arginase
• EC 3.5.3.11: agmatinase
• EC 3.5.3.12: agmatine deiminase
• EC 3.5.3.13: formimidoylglutamate deiminase
• EC 3.5.3.14: amidinoaspartase
• EC 3.5.3.15: protein-arginine deiminase
• EC 3.5.3.16: methylguanidinase
• EC 3.5.3.17: Guanidinopropionase
• EC 3.5.3.18: dimethylargininase
• EC 3.5.3.19: ureidoglycolate hydrolase
• EC 3.5.3.20: diguanidinobutanase
• EC 3.5.3.21: Methylenediurea deaminase
• EC 3.5.3.22: proclavaminate amidinohydrolase
• EC 3.5.3.23: N-succinylarginine dihydrolase

3.5.4: cyclic amidines[edit]

• EC 3.5.4.1: cytosine deaminase
• EC 3.5.4.2: adenine deaminase
• EC 3.5.4.3: guanine deaminase
• EC 3.5.4.4: adenosine deaminase
• EC 3.5.4.5: cytidine deaminase
• EC 3.5.4.6: AMP deaminase
• EC 3.5.4.7: ADP deaminase
• EC 3.5.4.8: aminoimidazolase
• EC 3.5.4.9: methenyltetrahydrofolate cyclohydrolase
• EC 3.5.4.10: IMP cyclohydrolase
• EC 3.5.4.11: pterin deaminase
• EC 3.5.4.12: dCMP deaminase
• EC 3.5.4.13: dCTP deaminase
• EC 3.5.4.14: deoxycytidine deaminase
• EC 3.5.4.15: guanosine deaminase
• EC 3.5.4.16: GTP cyclohydrolase I
• EC 3.5.4.17: adenosine-phosphate deaminase
• EC 3.5.4.18: ATP deaminase
• EC 3.5.4.19: phosphoribosyl-AMP cyclohydrolase
• EC 3.5.4.20: pyrithiamine deaminase
• EC 3.5.4.21: creatinine deaminase
• EC 3.5.4.22: 1-pyrroline-4-hydroxy-2-carboxylate deaminase
• EC 3.5.4.23: blasticidin-S deaminase
• EC 3.5.4.24: sepiapterin deaminase
• EC 3.5.4.25: GTP cyclohydrolase II
• EC 3.5.4.26: diaminohydroxyphosphoribosylaminopyrimidine deaminase
• EC 3.5.4.27: methenyltetrahydromethanopterin cyclohydrolase
• EC 3.5.4.28: S-adenosylhomocysteine deaminase
• EC 3.5.4.29: GTP cyclohydrolase IIa
• EC 3.5.4.30: dCTP deaminase (dUMP-forming)
• EC 3.5.4.31: S-methyl-5'-thioadenosine deaminase
• EC 3.5.4.32: 8-oxoguanine deaminase

3.5.5: nitriles[edit]

• EC 3.5.5.1: nitrilase
• EC 3.5.5.2: ricinine nitrilase
• EC 3.5.5.3: Transferred entry: cyanate hydrolase. Now EC 4.2.1.104, cyanate hydratase
• EC 3.5.5.4: cyanoalanine nitrilase
• EC 3.5.5.5: arylacetonitrilase
• EC 3.5.5.6: bromoxynil nitrilase
• EC 3.5.5.7: aliphatic nitrilase
• EC 3.5.5.8: thiocyanate hydrolase

3.5.99: other compounds[edit]

• EC 3.5.99.1: riboflavinase
• EC 3.5.99.2: aminopyrimidine aminohydrolase
• EC 3.5.99.3: hydroxydechloroatrazine ethylaminohydrolase
• EC 3.5.99.4: N-isopropylammelide isopropylaminohydrolase
• EC 3.5.99.5: 2-aminomuconate deaminase
• EC 3.5.99.6: glucosamine-6-phosphate deaminase
• EC 3.5.99.7: 1-aminocyclopropane-1-carboxylate deaminase
• EC 3.5.99.8: 5-nitroanthranilic acid aminohydrolase
• EC 3.5.99.9: 2-nitroimidazole nitrohydrolase

EC 3.6: act on acid anhydrides[edit]

3.6.1[edit]

• EC 3.6.1.1: inorganic diphosphatase
• EC 3.6.1.2: trimetaphosphatase
• EC 3.6.1.3: adenosinetriphosphatase
• EC 3.6.1.4: Deleted entry: adenosinetriphosphatase (Mg-activated). Now included with EC 3.6.1.3 adenosinetriphosphatase
• EC 3.6.1.5: apyrase
• EC 3.6.1.6: nucleoside-diphosphatase
• EC 3.6.1.7: acylphosphatase
• EC 3.6.1.8: ATP diphosphatase
• EC 3.6.1.9: nucleotide diphosphatase
• EC 3.6.1.10: endopolyphosphatase
• EC 3.6.1.11: exopolyphosphatase
• EC 3.6.1.12: dCTP diphosphatase
• EC 3.6.1.13: ADP-ribose diphosphatase
• EC 3.6.1.14: adenosine-tetraphosphatase
• EC 3.6.1.15: nucleoside-triphosphatase
• EC 3.6.1.16: CDP-glycerol diphosphatase
• EC 3.6.1.17: bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
• EC 3.6.1.18: FAD diphosphatase
• EC 3.6.1.19: nucleoside-triphosphate diphosphatase
• EC 3.6.1.20: 5'-acylphosphoadenosine hydrolase
• EC 3.6.1.21: ADP-sugar diphosphatase
• EC 3.6.1.22: NAD+ diphosphatase
• EC 3.6.1.23: dUTP diphosphatase
• EC 3.6.1.24: nucleoside phosphoacylhydrolase
• EC 3.6.1.25: triphosphatase
• EC 3.6.1.26: CDP-diacylglycerol diphosphatase
• EC 3.6.1.27: undecaprenyl-diphosphatase
• EC 3.6.1.28: thiamine-triphosphatase
• EC 3.6.1.29: bis(5'-adenosyl)-triphosphatase
• EC 3.6.1.30: m7G(5')pppN diphosphatase
• EC 3.6.1.31: phosphoribosyl-ATP diphosphatase
• EC 3.6.1.32: Transferred entry: myosin ATPase. Now EC 3.6.4.1, myosin ATPase
• EC 3.6.1.33: Transferred entry: dynein ATPase. Now EC 3.6.4.2, dynein ATPase
• EC 3.6.1.34: Transferred entry: H⁺-transporting ATP synthase. Now EC 3.6.3.14, H⁺-transporting two-sector ATPase
• EC 3.6.1.35: Transferred entry: H⁺-transporting ATPase. Now EC 3.6.3.6, H⁺-exporting ATPase
• EC 3.6.1.36: Transferred entry: H⁺/K⁺ exchanging ATPase. Now EC 3.6.3.10, H⁺/K⁺-exchanging ATPase
• EC 3.6.1.37: Transferred entry: Na⁺/K⁺ exchanging ATPase. Now EC 3.6.3.9, Na⁺/K⁺-exchanging ATPase
• EC 3.6.1.38: Transferred entry: Ca²⁺-transporting ATPase. Now EC 3.6.3.8, Ca²⁺-transporting ATPase
• EC 3.6.1.39: thymidine-triphosphatase
• EC 3.6.1.40: guanosine-5'-triphosphate,3'-diphosphate diphosphatase
• EC 3.6.1.41: bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
• EC 3.6.1.42: guanosine-diphosphatase
• EC 3.6.1.43: dolichyldiphosphatase
• EC 3.6.1.44: oligosaccharide-diphosphodolichol diphosphatase
• EC 3.6.1.45: UDP-sugar diphosphatase
• EC 3.6.1.46: Transferred entry: heterotrimeric G-protein GTPase. Now EC 3.6.5.1, heterotrimeric G-protein GTPase
• EC 3.6.1.47: Transferred entry: small monomeric GTPase. Now EC 3.6.5.2, small monomeric GTPase
• EC 3.6.1.48: Transferred entry: protein-synthesizing GTPase. Now EC 3.6.5.3, protein-synthesizing GTPase
• EC 3.6.1.49: Transferred entry: signal-recognition-particle GTPase. Now EC 3.6.5.4, signal-recognition-particle GTPase
• EC 3.6.1.50: Transferred entry: dynamin GTPase. Now EC 3.6.5.5, dynamin GTPase
• EC 3.6.1.51: Transferred entry: tubulin GTPase. Now EC 3.6.5.6, tubulin GTPase
• EC 3.6.1.52: diphosphoinositol-polyphosphate diphosphatase
• EC 3.6.1.53: Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase
• EC 3.6.1.54: UDP-2,3-diacylglucosamine diphosphatase
• EC 3.6.1.55: 8-oxo-dGTP diphosphatase
• EC 3.6.1.56: 2-hydroxy-dATP diphosphatase
• EC 3.6.1.57: UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase
• EC 3.6.1.58: 8-oxo-dGDP phosphatase
• EC 3.6.1.59: m7GpppX diphosphatase
• EC 3.6.1.60: diadenosine hexaphosphate hydrolase (AMP-forming)
• EC 3.6.1.61: diadenosine hexaphosphate hydrolase (ATP-forming)
• EC 3.6.1.62: m7GpppN-mRNA hydrolase
• EC 3.6.1.63: alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase

3.6.2[edit]

• EC 3.6.2.1: adenylylsulfatase
• EC 3.6.2.2: phosphoadenylylsulfatase

3.6.3[edit]

• EC 3.6.3.1: phospholipid-translocating ATPase
• EC 3.6.3.2: Mg2+-importing ATPase
• EC 3.6.3.3: Cd2+-exporting ATPase
• EC 3.6.3.4: Cu2+-exporting ATPase
• EC 3.6.3.5: Zn2+-exporting ATPase
• EC 3.6.3.6: H+-exporting ATPase
• EC 3.6.3.7: Na+-exporting ATPase
• EC 3.6.3.8: Ca2+-transporting ATPase
• EC 3.6.3.9: Na+/K+-exchanging ATPase
• EC 3.6.3.10: H+/K+-exchanging ATPase
• EC 3.6.3.11: Cl-transporting ATPase
• EC 3.6.3.12: K+-transporting ATPase
• EC 3.6.3.14: H+-transporting two-sector ATPase
• EC 3.6.3.15: Na+-transporting two-sector ATPase
• EC 3.6.3.16: arsenite-transporting ATPase
• EC 3.6.3.17: monosaccharide-transporting ATPase
• EC 3.6.3.18: oligosaccharide-transporting ATPase
• EC 3.6.3.19: maltose-transporting ATPase
• EC 3.6.3.20: glycerol-3-phosphate-transporting ATPase
• EC 3.6.3.21: polar-amino-acid-transporting ATPase
• EC 3.6.3.22: nonpolar-amino-acid-transporting ATPase
• EC 3.6.3.23: oligopeptide-transporting ATPase
• EC 3.6.3.24: nickel-transporting ATPase
• EC 3.6.3.25: sulfate-transporting ATPase
• EC 3.6.3.26: nitrate-transporting ATPase
• EC 3.6.3.27: phosphate-transporting ATPase
• EC 3.6.3.28: phosphonate-transporting ATPase
• EC 3.6.3.29: molybdate-transporting ATPase
• EC 3.6.3.30: Fe3+-transporting ATPase
• EC 3.6.3.31: polyamine-transporting ATPase
• EC 3.6.3.32: quaternary-amine-transporting ATPase
• EC 3.6.3.33: vitamin B12-transporting ATPase
• EC 3.6.3.34: iron-chelate-transporting ATPase
• EC 3.6.3.35: manganese-transporting ATPase
• EC 3.6.3.36: taurine-transporting ATPase
• EC 3.6.3.37: guanine-transporting ATPase
• EC 3.6.3.38: capsular-polysaccharide-transporting ATPase
• EC 3.6.3.39: lipopolysaccharide-transporting ATPase
• EC 3.6.3.40: teichoic-acid-transporting ATPase
• EC 3.6.3.41: heme-transporting ATPase
• EC 3.6.3.42: beta-glucan-transporting ATPase
• EC 3.6.3.43: peptide-transporting ATPase
• EC 3.6.3.44: xenobiotic-transporting ATPase
• EC 3.6.3.45: steroid-transporting ATPase
• EC 3.6.3.46: cadmium-transporting ATPase
• EC 3.6.3.47: fatty-acyl-CoA-transporting ATPase
• EC 3.6.3.48: alpha-factor-transporting ATPase
• EC 3.6.3.49: channel-conductance-controlling ATPase
• EC 3.6.3.50: protein-secreting ATPase
• EC 3.6.3.51: mitochondrial protein-transporting ATPase
• EC 3.6.3.52: chloroplast protein-transporting ATPase
• EC 3.6.3.53: Ag+-exporting ATPase

3.6.4[edit]

• EC 3.6.4.1: myosin ATPase
• EC 3.6.4.2: dynein ATPase
• EC 3.6.4.3: microtubule-severing ATPase
• EC 3.6.4.4: plus-end-directed kinesin ATPase
• EC 3.6.4.5: minus-end-directed kinesin ATPase
• EC 3.6.4.6: vesicle-fusing ATPase
• EC 3.6.4.7: peroxisome-assembly ATPase
• EC 3.6.4.8: proteasome ATPase
• EC 3.6.4.9: chaperonin ATPase
• EC 3.6.4.10: non-chaperonin molecular chaperone ATPase
• EC 3.6.4.11: nucleoplasmin ATPase
• EC 3.6.4.12: DNA helicase
• EC 3.6.4.13: RNA helicase

3.6.5[edit]

• EC 3.6.5.1: heterotrimeric G-protein GTPase
• EC 3.6.5.2: small monomeric GTPase
• EC 3.6.5.3: protein-synthesizing GTPase
• EC 3.6.5.4: signal-recognition-particle GTPase
• EC 3.6.5.5: dynamin GTPase
• EC 3.6.5.6: tubulin GTPase

EC 3.7: act on carbon-carbon bonds[edit]

• EC 3.7.1.1: oxaloacetase
• EC 3.7.1.2: fumarylacetoacetase
• EC 3.7.1.3: kynureninase
• EC 3.7.1.4: phloretin hydrolase
• EC 3.7.1.5: acylpyruvate hydrolase
• EC 3.7.1.6: acetylpyruvate hydrolase
• EC 3.7.1.7: beta-diketone hydrolase
• EC 3.7.1.8: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase
• EC 3.7.1.9: 2-hydroxymuconate-semialdehyde hydrolase
• EC 3.7.1.10: cyclohexane-1,3-dione hydrolase
• EC 3.7.1.11: cyclohexane-1,2-dione hydrolase
• EC 3.7.1.12: cobalt-precorrin 5A hydrolase
• EC 3.7.1.13: 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase
• EC 3.7.1.14: 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
• EC 3.7.1.15: (+)-caryolan-1-ol synthase
• EC 3.7.1.16: oxepin-CoA hydrolase
• EC 3.7.1.17: 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
• EC 3.7.1.18: 6-oxocamphor hydrolase
• EC 3.7.1.19: 2,6-dihydroxypseudooxynicotine hydrolase
• EC 3.7.1.20: 3-fumarylpyruvate hydrolase

EC 3.8: act on halide bonds[edit]

• EC 3.8.1.1: alkylhalidase
• EC 3.8.1.2: 2-haloacid dehalogenase
• EC 3.8.1.3: haloacetate dehalogenase
• EC 3.8.1.4: thyroxine deiodinase
• EC 3.8.1.5: haloalkane dehalogenase
• EC 3.8.1.6: 4-chlorobenzoate dehalogenase
• EC 3.8.1.7: 4-chlorobenzoyl-CoA dehalogenase
• EC 3.8.1.8: atrazine chlorohydrolase
• EC 3.8.1.9: (R)-2-haloacid dehalogenase
• EC 3.8.1.10: 2-haloacid dehalogenase (configuration-inverting)
• EC 3.8.1.11: 2-haloacid dehalogenase (configuration-retaining)

• EC 3.8.2.1: Transferred entry: di-isopropyl-fluorophosphatase. Now EC 3.1.8.2, diisopropyl-fluorophosphatase

EC 3.9: act on phosphorus-nitrogen bonds[edit]

• EC 3.9.1.1: phosphoamidase

EC 3.10: act on sulfur-nitrogen bonds[edit]

• EC 3.10.1.1: N-sulfoglucosamine sulfohydrolase
• EC 3.10.1.2: cyclamate sulfohydrolase

EC 3.11: act on carbon-phosphorus bonds[edit]

• EC 3.11.1.1: phosphonoacetaldehyde hydrolase
• EC 3.11.1.2: phosphonoacetate hydrolase
• EC 3.11.1.3: phosphonopyruvate hydrolase

EC 3.12: act on sulfur-sulfur bonds[edit]

• EC 3.12.1.1: trithionate hydrolase

EC 3.13: act on carbon-sulfur bonds[edit]

• EC 3.13.1.1: UDP-sulfoquinovose synthase
• EC 3.13.1.2: Deleted entry: 5-deoxyribos-5-ylhomocysteinase. The activity is most probably attributable to EC 4.4.1.21, S-ribosylhomocysteine lyase
• EC 3.13.1.3: 2'-hydroxybiphenyl-2-sulfinate desulfinase

v t e Proteins: enzymes Topics Active site Allosteric regulation Binding site Catalytically perfect enzyme Coenzyme Cofactor Cooperativity EC number Enzyme catalysis Enzyme inhibitor Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics Enzyme promiscuity List of enzymes Types EC1 Oxidoreductases/list EC2 Transferases/list EC3 Hydrolases/list EC4 Lyases/list EC5 Isomerases/list EC6 Ligases/list B enzm 1.1 2 3 4 5 6 7 8 10 11 13 14 15-18 2.1 2 3 4 5 6 7 2.7.10 11-12 8 3.1 3.1.3.48 2 3 4 3.4.21 22 23 24 5 6 7 4.1 2 3 4 5 6 5.1 2 3 4 99 6.1-3 4 5-6

v t e Hydrolase: esterases (EC 3.1) 3.1.1: Carboxylic ester hydrolases Cholinesterase Acetylcholinesterase Butyrylcholinesterase Pectinesterase 6-phosphogluconolactonase PAF acetylhydrolase Lipase Bile salt-dependent Gastric/Lingual Pancreatic Lysosomal Hormone-sensitive Endothelial Hepatic Lipoprotein Monoacylglycerol Diacylglycerol Phospholipase A1 A2 B 3.1.2: Thioesterase Palmitoyl protein thioesterase Ubiquitin carboxy-terminal hydrolase L1 3.1.3: Phosphatase Alkaline phosphatase ALPI ALPL ALPP Acid phosphatase (Prostatic)/Tartrate-resistant acid phosphatase/Purple acid phosphatases Nucleotidase Glucose 6-phosphatase Fructose 1,6-bisphosphatase Calcineurin Phosphoprotein phosphatase PP2A OCRL Pyruvate dehydrogenase phosphatase Fructose 6-P,2-kinase:fructose 2,6-bisphosphatase PTEN Phytase Inositol-phosphate phosphatase IMPA1, IMPA2, IMPA3 Phosphoprotein phosphatase: Protein tyrosine phosphatase Protein serine/threonine phosphatase Dual-specificity phosphatase 3.1.4: Phosphodiesterase Autotaxin Phospholipase C D Sphingomyelin phosphodiesterase 1 PDE1 PDE2 PDE3 PDE4A/PDE4B PDE5 Lecithinase (Clostridium perfringens alpha toxin) Cyclic nucleotide phosphodiesterase 3.1.6: Sulfatase arylsulfatase Arylsulfatase A Arylsulfatase B Arylsulfatase E Steroid sulfatase Galactosamine-6 sulfatase Iduronate-2-sulfatase N-acetylglucosamine-6-sulfatase Nuclease (includes deoxyribonuclease and ribonuclease) 3.1.11-16: Exonuclease Exodeoxyribonuclease RecBCD Exoribonuclease Oligonucleotidase 3.1.21-31: Endonuclease Endodeoxyribonuclease Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme UvrABC endonuclease Endoribonuclease RNase III RNase H 1 2A 2B 2C RNase P RNase A 1 2 3 4/5 RNase T1 RNA-induced silencing complex either deoxy- or ribo- Aspergillus nuclease S1 Micrococcal nuclease B enzm 1.1 2 3 4 5 6 7 8 10 11 13 14 15-18 2.1 2 3 4 5 6 7 2.7.10 11-12 8 3.1 3.1.3.48 2 3 4 3.4.21 22 23 24 5 6 7 4.1 2 3 4 5 6 5.1 2 3 4 99 6.1-3 4 5-6

v t e Hydrolase: sugar hydrolases (EC 3.2) 3.2.1: Glycoside hydrolases Disaccharidase Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase Glucosidases Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme Other Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2 3.2.2: Hydrolysing N-Glycosyl compounds DNA glycosylases: Oxoguanine glycosylase B enzm 1.1 2 3 4 5 6 7 8 10 11 13 14 15-18 2.1 2 3 4 5 6 7 2.7.10 11-12 8 3.1 3.1.3.48 2 3 4 3.4.21 22 23 24 5 6 7 4.1 2 3 4 5 6 5.1 2 3 4 99 6.1-3 4 5-6

v t e Hydrolases: ether bond (EC 3.3) 3.3.1 Adenosylhomocysteinase 3.3.2 Epoxide hydrolase Leukotriene-A4 hydrolase B enzm 1.1 2 3 4 5 6 7 8 10 11 13 14 15-18 2.1 2 3 4 5 6 7 2.7.10 11-12 8 3.1 3.1.3.48 2 3 4 3.4.21 22 23 24 5 6 7 4.1 2 3 4 5 6 5.1 2 3 4 99 6.1-3 4 5-6

v t e Hydrolase: proteases (EC 3.4) 3.4.11-19: Exopeptidase 3.4.11 Aminopeptidase Alanine Arginyl Aspartyl Cystinyl Leucyl Glutamyl Methionyl 1 2 O 3.4.13 Dipeptidase 1 2 3 3.4.14 Dipeptidyl peptidase Cathepsin C Dipeptidyl peptidase-4 Tripeptidyl peptidase Tripeptidyl peptidase I Tripeptidyl peptidase II 3.4.15 Angiotensin-converting enzyme 3.4.16 Serine type carboxypeptidases: Cathepsin A DD-transpeptidase 3.4.17 Metallocarboxypeptidases: Carboxypeptidase A A2 B C E Glutamate II Other/ungrouped Metalloexopeptidase 3.4.21-24: Endopeptidase Serine proteases Cysteine protease Aspartic acid protease Metalloendopeptidases Other/ungrouped: Amyloid precursor protein secretase Alpha secretase Beta-secretase 1 Beta-secretase 2 Gamma secretase 3.4.99: Unknown Staphylokinase B enzm 1.1 2 3 4 5 6 7 8 10 11 13 14 15-18 2.1 2 3 4 5 6 7 2.7.10 11-12 8 3.1 3.1.3.48 2 3 4 3.4.21 22 23 24 5 6 7 4.1 2 3 4 5 6 5.1 2 3 4 99 6.1-3 4 5-6

v t e Hydrolases: carbon-nitrogen non-peptide (EC 3.5) 3.5.1: Linear amides / Amidohydrolases Asparaginase Glutaminase Urease Biotinidase Aspartoacylase Ceramidase Aspartylglucosaminidase Fatty acid amide hydrolase Histone deacetylase Sirtuin 3.5.2: Cyclic amides/ Amidohydrolases Barbiturase Beta-lactamase 3.5.3: Linear amidines/ Ureohydrolases Arginase Agmatinase Protein-arginine deiminase 3.5.4: Cyclic amidines/ Aminohydrolases Guanine deaminase Adenosine deaminase AMP deaminase Inosine monophosphate synthase DCMP deaminase GTP cyclohydrolase I Cytidine deaminase AICDA Activation-Induced (Cytidine) Deaminase 3.5.5: Nitriles/ Aminohydrolases Nitrilase 3.5.99: Other Riboflavinase Thiaminase II B enzm 1.1 2 3 4 5 6 7 8 10 11 13 14 15-18 2.1 2 3 4 5 6 7 2.7.10 11-12 8 3.1 3.1.3.48 2 3 4 3.4.21 22 23 24 5 6 7 4.1 2 3 4 5 6 5.1 2 3 4 99 6.1-3 4 5-6

v t e Hydrolases: acid anhydride hydrolases (EC 3.6) 3.6.1 Pyrophosphatase Inorganic Thiamine Apyrase Thiamine-triphosphatase 3.6.2 Adenylylsulfatase Phosphoadenylylsulfatase 3.6.3-4: ATPase 3.6.3 Cu++ (3.6.3.4) Menkes/ATP7A Wilson/ATP7B Ca+ (3.6.3.8) SERCA ATP2A1 ATP2A2 ATP2A3 Plasma membrane ATP2B1 ATP2B2 ATP2B3 ATP2B4 SPCA ATP2C1 ATP2C2 Na+/K+ (3.6.3.9) ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 ATP1B4 H+/K+ (3.6.3.10) ATP4A Other P-type ATPase ATP8B1 ATP10A ATP11B ATP12A ATP13A2 ATP13A3 3.6.4 Dynein Kinesin Myosin Katanin 3.6.5: GTPase 3.6.5.1: Heterotrimeric G protein Gαs Gαi GNAI1 GNAI2 GNAI3 Gαq/11 GNAQ GNA11 Gα12/13 GNA12 GNA13 Transducin GNAT1 GNAT2 3.6.5.2: Small GTPase > Ras superfamily Rho family of GTPases: Cdc42 CDC42 TC10 TCL RhoUV RhoU RhoV Rac Rac1 2 3 RhoG RhoBTB 1 2 RhoH Rho A B C Rnd 1 2 3 RhoDF RhoF RhoD other: Ras HRAS KRAS NRAS Rab RAB23 RAB27 Arf ARF6 SAR1B ARL13B ARL6 Ran Rheb Rap 3.6.5.3: Protein-synthesizing GTPase Prokaryotic IF-2 EF-Tu EF-G Eukaryotic 3.6.5.5-6: Polymerization motors Dynamin Tubulin B enzm 1.1 2 3 4 5 6 7 8 10 11 13 14 15-18 2.1 2 3 4 5 6 7 2.7.10 11-12 8 3.1 3.1.3.48 2 3 4 3.4.21 22 23 24 5 6 7 4.1 2 3 4 5 6 5.1 2 3 4 99 6.1-3 4 5-6

v t e Hydrolases: carbon-carbon (EC 3.7) 3.7.1 Fumarylacetoacetate hydrolase Kynureninase B enzm 1.1 2 3 4 5 6 7 8 10 11 13 14 15-18 2.1 2 3 4 5 6 7 2.7.10 11-12 8 3.1 3.1.3.48 2 3 4 3.4.21 22 23 24 5 6 7 4.1 2 3 4 5 6 5.1 2 3 4 99 6.1-3 4 5-6