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Isoamyl alcohol[1]
Isoamyl alcohol.svg
CAS number 123-51-3 YesY
PubChem 31260
ChemSpider 29000 YesY
DrugBank DB02296
KEGG C07328 YesY
ChEBI CHEBI:15837 YesY
Jmol-3D images Image 1
Molecular formula C5H12O
Molar mass 88.148 g/mol
Appearance Clear, colorless liquid
Density 0.8104 g/cm3 at 20 °C
Melting point −117.2 °C (−179.0 °F; 156.0 K)
Boiling point 131.1 °C (268.0 °F; 404.2 K)
Solubility in water slightly soluble, 28 g/L
Solubility very soluble in acetone, diethyl ether, ethanol
Viscosity 3.692 mPa·s
2.382 J·g-1·K-1
-356.4 kJ·mol-1 (liquid)
-300.7 kJ·mol-1 (gas)
Flash point 43 °C (109 °F; 316 K)
Autoignition temperature 350 °C (662 °F; 623 K)
Explosive limits 1.2 – 9%
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 YesY (verify) (what is: YesY/N?)
Infobox references

Isoamyl alcohol (also known as isopentyl alcohol) is a clear, colorless alcohol with the formula (CH3)2CHCH2CH2OH. It is one of several isomers of amyl alcohol. It is a main ingredient in the production of banana oil, an ester found in nature and also produced as a flavouring in industry. It's also the main ingredient of Kovac's reagent, used for the bacterial diagnostic indole test.

It is also used as an antifoaming agent in the Chloroform:Isomyl Alcohol reagent.[2]

Isoamyl alcohol is used in a Phenol–chloroform extraction mixed with the chloroform to further inhibit RNase activity and prevent solubility of RNAs with long tracts of poly-adenine.[3]

It is one of the components of the aroma of Tuber melanosporum, the black truffle. It has been identified as a chemical in the pheromone used by hornets to attract other members of the hive to attack.[citation needed]


  1. ^ Lide, David R., ed. (1998). Handbook of Chemistry and Physics (87 ed.). Boca Raton, FL: CRC Press. pp. 3–374, 5–42, 6–188, 8–102, 15–22. ISBN 0-8493-0487-3. 
  2. ^ Zumbo, P. "Phenol-chloroform Extraction". WEILL CORNELL MEDICAL COLLEGE P. ZUMBO LABORATORY OF CHRISTOPHER E. MASON, PH.D. Retrieved 19 June 2014. 
  3. ^ Green, Michael; Sambrook, Joseph. "Purification of Nucleic Acids: Extraction with Phenol-Chloroform". Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. ISBN 1936113422. 

Original courtesy of Wikipedia: http://en.wikipedia.org/wiki/Isoamyl_alcohol — Please support Wikipedia.
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73 videos foundNext > 

Isoamyl acetate

Isoamyl acetate.

Esterification (Banana Oil)

Esterification of acetic acid with isopentyl alcohol to make isopentyl acetate.

Plasmid Isolation for Bacteria

Reagents and solutions Alkaline Solution I 50 mM glucose 25 mM Tris HCl (pH 8.0) 10 mM EDTA (pH 8.0) 10 mg/ml lysozyme Alkaline Solution II 0.2 N NaOH (2.0 m...

The Chemistry of Alcohol and Hangovers - Bytesize Science

Saint Patrick's day is this Sunday, and there are many ways to celebrate like Irish soda bread at breakfast or corned beef and cabbage for dinner. For those ...

Sperian Protection Air Purifying Respirator Fit Testing: Isoamyl Acetate (Banana Oil)

Instructions for fit testing air purifying respirators with isoamyl acetate (banana oil).

Synthesis of Fragrant Esters

By Anthony Ogugu PS: the isoamyl alcohol had broken in my science kit and consequently I was not able to use it in this lab. Sorry.

Điều chế isoamyl axetat (Lê Văn Đăng_GV Khoa Hóa Học_Trường ĐHSP TP.HCM)

Cách tiến hành : 1-Giai đoạn điều chế isoamyl axetat (thời gian khoảng 1 giờ) : Cho 12,5 gam natri axetat khan đã nghiền nhỏ vào bình Vuyếc dung tích 100ml, ...

DNA Extraction by Phenol Chloroform

http://www.abnova.com ) - This method relies on phase separation by centrifugation of a mix of the aqueous sample and a solution containing phenol and chlo...


Today we are going to go over the esterification of acetic acid with isopentyl alcohol. This presentation looks in detail at the mechanism for the formation ...

What does isoamyl nitrite mean?

What does isoamyl nitrite mean? A spoken definition of isoamyl nitrite. Intro Sound: Typewriter - Tamskp Licensed under CC:BA 3.0 Outro Music: Groove Groove ...

73 videos foundNext > 

14 news items

Ethanol Producer Magazine

Ethanol Producer Magazine
Tue, 14 Oct 2014 07:10:35 -0700

... list of VOCs from ethanol production has emerged, some common VOCs tested for include acetaldehyde, acetic acid, acetoin, acrolein, 2,3-butanediol, 2,3-butanedione, ethanol, ethyl acetate, formaldehyde, 2-furaldehyde, isoamyl alcohol and methanol.

SPIE Newsroom

SPIE Newsroom
Tue, 09 Dec 2014 08:30:00 -0800

Live worm in a microfluidic chamber with Archer1 expressing in one amphid wing cell (AWC) neuron. The intensity of Archer1's red fluorescence can be used to track the AWC's electrical activity in response to stimulation with odorants such as isoamyl ...

Ars Technica

Ars Technica
Sat, 05 Jul 2014 05:00:00 -0700

... the samples "were thoroughly cleaned to remove surface contamination, ground into a buffer solution in a glass homogenizer then incubated for 2 h at 56ºC in a solution containing proteinase K before extraction with phenol/chloroform/isoamyl alcohol.
Mon, 24 Feb 2014 03:23:50 -0800

Several compounds differed between cancer and normal cells, e.g., isoamyl alcohol was higher in melanoma cells than in normal melanocytes but isovaleric acid was lower in melanoma cells. These two compounds share the same precursor, viz., leucine.

Medical Daily

Medical Daily
Thu, 13 Jun 2013 20:26:48 -0700

In this case, the nanotube sensors were able to detect significant levels of airborne compounds associated with melanoma cells, like isoamyl alcohol, which typically has a sickeningly sweet smell in high concentrations, and dimethyl disulfide, which is ...


Mon, 12 Aug 2013 07:48:45 -0700

After testing isoamyl alcohol, n-butanol, n-octanol and n-hexane, the first two solvents dissolved all ten additives with similar recoveries but n-butanol gave less HPLC interference so was chosen as the preferred solvent. The solution was adjusted to ...
Sat, 11 May 2013 03:58:02 -0700

After a single extraction, the majority of the free dye partitioned to the butanol phase; labeled and unlabeled DNA remained in the aqueous phase throughout the extraction process. We also tested another water-saturated solvent, isoamyl alcohol, and ...
Thu, 21 Jun 2012 16:37:30 -0700

A solution of 600 μl phenol:chloroform:isoamyl alcohol (25:24:1) was then added. The samples were subjected to pressure cycling at 35 000 psi for 20 s and 0 psi for 10 s for a total of 20 cycles using the Barocycler NEP3229 (Pressure Biosciences).

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