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Erythropoietin
Erythropoietin.png
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols EPO ; EP; MVCD2
External IDs OMIM133170 MGI95407 HomoloGene624 ChEMBL: 5837 GeneCards: EPO Gene
RNA expression pattern
PBB GE EPO 207257 at tn.png
PBB GE EPO 217254 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 2056 13856
Ensembl ENSG00000130427 ENSMUSG00000029711
UniProt P01588 P07321
RefSeq (mRNA) NM_000799 NM_007942
RefSeq (protein) NP_000790 NP_031968
Location (UCSC) Chr 7:
100.32 – 100.32 Mb
Chr 5:
137.48 – 137.53 Mb
PubMed search [1] [2]

Erythropoietin, (/ɨˌrɪθrɵˈpɔɪ.ɨtɨn/) also known as EPO, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine (protein signaling molecule) for erythrocyte (red blood cell) precursors in the bone marrow. Human EPO has a molecular weight of 34 kDa.

Also called hematopoietin or hemopoietin, it is produced by interstitial fibroblasts in the kidney in close association with peritubular capillary and tubular epithelial tubule. It is also produced in perisinusoidal cells in the liver. While liver production predominates in the fetal and perinatal period, renal production is predominant during adulthood. In addition to erythropoiesis, erythropoietin also has other known biological functions. For example, it plays an important role in the brain's response to neuronal injury.[1] EPO is also involved in the wound healing process.[2]

Exogenous erythropoietin is produced by recombinant DNA technology in cell culture. Several different pharmaceutical agents are available with a variety of glycosylation patterns, and are collectively called erythropoiesis-stimulating agents (ESA). The specific details for labelled use vary between the package inserts, but ESAs have been used in the treatment of anemia in chronic kidney disease, anemia in myelodysplasia, and in anemia from cancer chemotherapy. Boxed warnings include a risk of death, myocardial infarction, stroke, venous thromboembolism, and tumor recurrence.[3] Exogenous erythropoietin has been used illicitly as a performance-enhancing drug; it can often be detected in blood, due to slight differences from the endogenous protein, for example, in features of posttranslational modification.

Function[edit]

Red blood cell production[edit]

The primary role of erythropoietin is an essential hormone for red cell production. Without it, definitive erythropoiesis does not take place. Under hypoxic conditions, the kidney will produce and secrete erythropoietin to increase the production of red blood cells by targeting CFU-E, proerythroblast and basophilic erythroblast subsets in the differentiation. Erythropoietin has its primary effect on red blood cell progenitors and precursors (which are found in the bone marrow in humans) by promoting their survival through protecting these cells from apoptosis.

Erythropoietin is the primary erythropoietic factor that cooperates with various other growth factors (e.g., IL-3, IL-6, glucocorticoids, and SCF) involved in the development of erythroid lineage from multipotent progenitors. The burst-forming unit-erythroid (BFU-E) cells start erythropoietin receptor expression and are sensitive to erythropoietin. Subsequent stage, the colony-forming unit-erythroid (CFU-E), expresses maximal erythropoietin receptor density and is completely dependent on erythropoietin for further differentiation. Precursors of red cells, the proerythroblasts and basophilic erythroblasts also express erythropoietin receptor and are therefore affected by it.

Nonhematopoietic roles[edit]

Erythropoietin has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. It can increase iron absorption by suppressing the hormone hepcidin.[4]

EPO levels of 100 times the baseline have been detected in brain tissue as a natural response to hypoxic damage.[5] In rats, pretreatment with erythropietin was associated with neuronal protection during induced cerebral hypoxia.[6] Trials in humans have not been reported.

Multiple studies have suggested that EPO improves memory. This effect is independent of its effect on hematocrit.[7][8] Rather, it is associated with an increase in hippocampal response and effects on synaptic connectivity, neuronal plasticity, and memory-related neural networks.[9][10] EPO may have effects on mood.[8][11]

Mechanism of action[edit]

Erythropoietin has been shown to exert its effects by binding to the erythropoietin receptor (EpoR).[12][13]

EPO is highly glycosylated (40% of total molecular weight), with half-life in blood around five hours. EPO's half-life may vary between endogenous and various recombinant versions. Additional glycosylation or other alterations of EPO via recombinant technology have led to the increase of EPO's stability in blood (thus requiring less frequent injections). EPO binds to the erythropoietin receptor on the red cell progenitor surface and activates a JAK2 signaling cascade. Erythropoietin receptor expression is found in a number of tissues, such as bone marrow and peripheral/central nervous tissue. In the bloodstream, red cells themselves do not express erythropoietin receptor, so cannot respond to EPO. However, indirect dependence of red cell longevity in the blood on plasma erythropoietin levels has been reported, a process termed neocytolysis.

Synthesis and regulation[edit]

Erythropoietin levels in blood are quite low in the absence of anemia, at around 10 mU/ml. However, in hypoxic stress, EPO production may increase 1000-fold, reaching 10,000 mU/ml of blood. EPO is produced mainly by peritubular capillary lining cells of the renal cortex, which are highly specialized, epithelial-like cells. It is synthesized by renal peritubular cells in adults, with a small amount being produced in the liver.[14][15] Regulation is believed to rely on a feedback mechanism measuring blood oxygenation.[16] Constitutively synthesized transcription factors for EPO, known as hypoxia-inducible factors, are hydroxylated and proteosomally digested in the presence of oxygen.

Medical uses[edit]

Erythropoietins available for use as therapeutic agents are produced by recombinant DNA technology in cell culture, and include Epogen/Procrit (epoetin alfa) and Aranesp (darbepoetin alfa); they are used in treating anemia resulting from chronic kidney disease, inflammatory bowel disease (Crohn's disease and ulcer colitis) [17] and myelodysplasia from the treatment of cancer (chemotherapy and radiation). The package inserts include boxed warnings of increased risk of death, myocardial infarction, stroke, venous thromboembolism, and tumor recurrence, particularly when used to increase the hemoglobin levels to more than 11 to 12 g/dl.[3]

Available forms[edit]

Recombinant erythropoietin has a variety of glycosylation patterns giving rise to alfa, beta, delta, and omega forms:

  • epoetin zeta (biosimilar forms for epoetin alpha):
    • Silapo (Stada)
    • Retacrit (Hospira)
  • Miscellaneous:
    • Epocept, made by Lupin Pharmaceuticals
    • EPOTrust, made by Panacea Biotec Ltd
    • Erypro Safe, made by Biocon Ltd.
    • Repoitin, made by Serum Institute of India Limited
    • Vintor, made by Emcure Pharmaceuticals
    • Epofit, made by Intas pharma
    • Erykine, made by Intas Biopharmaceutica
    • Wepox, made by Wockhardt Biotech
    • Espogen, made by LG life sciences.
    • ReliPoietin, made by Reliance Life Sciences
    • Shanpoietin, made by Shantha Biotechnics Ltd
    • Zyrop, made by Cadila Healthcare Ltd.
    • EPIAO (rHuEPO), made by Shenyang Sunshine Pharmaceutical Co.. LTD. China
    • Cinnapoietin, made by CinnaGen biopharmaceutical Iran.

Darbepoetin alfa, which early literature during its development often termed as novel erythropoiesis-stimulating protein (NESP), is a form created by five substitutions (Asn-57, Thr-59, Val-114, Asn-115 and Thr-117) that create two new N-glycosylation sites.[20] This glycoprotein has a longer terminal half-life, meaning it may be possible to administer it less frequently.

Blood doping[edit]

Erythropoiesis-stimulating agents (ESAs) have a history of use as blood doping agents in endurance sports, such as horseracing, boxing,[21] cycling, rowing, distance running, race walking, snowshoeing, cross country skiing, biathlon, and triathlon. The overall oxygen delivery system (blood oxygen levels, as well as heart stroke volume, vascularization, and lung function) is one of the major limiting factors to muscles' ability to perform endurance exercise. Therefore, the primary reason athletes may use ESAs is to improve oxygen delivery to muscles, which directly improves their endurance capacity. With the advent of recombinant erythropoietin in the 1990s, the practice of autologous and homologous blood transfusion has been partially replaced by injecting erythropoietin such that the body naturally produces its own red cells. ESAs increase hematocrit (% of blood volume that is red cell mass) and total red cell mass in the body, providing a good advantage in sports where such practice is banned.[22] In addition to ethical considerations in sports, providing an increased red cell mass beyond the natural levels reduces blood flow due to increased viscosity, and increases the likelihood of thrombosis and stroke. Due to dangers associated with using ESAs, their use should be limited to the clinic where anemic patients are boosted back to normal hemoglobin levels (as opposed to going above the normal levels for performance advantage, leading to an increased risk of death).

Though EPO was believed to be widely used in the 1990s in certain sports, there was no way at the time to directly test for it, until in 2000, when a test developed by scientists at the French national antidoping laboratory (LNDD) and endorsed by the World Anti-Doping Agency (WADA) was introduced to detect pharmaceutical EPO by distinguishing it from the nearly identical natural hormone normally present in an athlete's urine. The first EPO-doping cases were found by the Swiss Laboratory for Doping Analyses.[23]

In 2002, at the Winter Olympic Games in Salt Lake City, Dr. Don Catlin, the founder and then-director of the UCLA Olympic Analytical Lab, reported finding darbepoetin alfa, a form of erythropoietin, in a test sample for the first time in sports.[24] At the 2012 Summer Olympics in London, Alex Schwazer, the gold medalist in the 50-kilometer race walk in the 2008 Summer Olympics in Beijing, tested positive for EPO and was disqualified.[25]

Since 2002, EPO tests performed by US sports authorities have consisted of only a urine or "direct" test. From 2000–2006, EPO tests at the Olympics were conducted on both blood and urine.[26][27] However, several compounds have been identified that can be taken orally to stimulate endogenous EPO production. Most of the compounds stabilize the hypoxia-inducible transcription factors which activate the EPO gene. The compounds include oxo-glutarate competitors, but also simple ions such as cobalt(II) chloride.[28]

Inhalation of a xenon/oxygen mixture activates production of the transcription factor HIF-1-alpha, which leads to increased production of erythropoietin and improved performance. It has been used for this purpose in Russia since at least 2004.[29]

Cycling[edit]

Synthetic EPO is believed to have come into use in cycling about 1990.[30] In theory, EPO use can increase VO2max by a significant amount,[31] making it useful for endurance sports like cycling. Italian antidoping advocate Sandro Donati has claimed that the history of doping in cycling can be traced to the Italian Dr Francesco Conconi at the University of Ferrara. Conconi had worked on the idea of giving athletes tranfusions of their own blood in the 1980s. Donati felt this work "opened the road to EPO . . . because blood doping was a trial to understand the role of EPO".[32]

Dr Michele Ferrari, a former student and mentee of Conconi,[33] had a controversial interview mentioning the drug in 1994, just after his Gewiss-Ballan team had a remarkable performance in the La Flèche Wallonne race. Ferrari told l'Equipe journalist Jean-Michel Rouet that EPO had no "fundamental" effect on performance and that if his riders used it, it wouldn't "scandalize" himself. After the journalist pointed out several riders were suspected of dying from EPO, Ferrari said EPO was not dangerous, and only abuse of it was dangerous, saying, "It's also dangerous to drink 10 liters of orange juice." The 'orange juice' comment has been widely misquoted.[34][35] Ferrari was fired shortly after, but continued to work in the industry with top riders that allegedly included Lance Armstrong.[33][36] That same year, Sandro Donati, working for the Italian National Olympic Committee, presented a report accusing Conconi of being linked to the use of EPO in the sport.[32]

In 1997, the Union Cycliste Internationale (UCI) instituted a new rule that riders testing above 50% haematocrit were not allowed to race.[37] Robert Millar, former racer, later wrote for Cycling News that the 50% limit was "an open invitation to dope to that level", pointing out that normally haematocrit levels would start "around 40-42%" and drop during the course of a "grand tour", but after EPO, they were staying at 50% for "weeks at a time".[38] By 1998, EPO use had become widespread, and the Festina affair tarnished the 1998 Tour de France.[30] One manager offered a 270,000-franc-per-month raise to Christophe Bassons if he would use EPO, but Bassons refused.[39]

In the 1998 Tour de France Stuart O'Grady won one stage, held the Tour de France yellow jersey for three days, and came second in the points classification with the assistance of EPO.[40] In 2010, Floyd Landis admitted to using performance-enhancing drugs, including EPO, throughout his career as a professional cyclist.[41] In 2012, the USADA released a report on its investigation into the US Postal Service cycling team and blood doping. The report contained affidavits from numerous riders on the team, including Frankie Andreu, Tyler Hamilton, George Hincapie, Floyd Landis, Levi Leipheimer, and others, outlining that they, and Lance Armstrong, used a cocktail of performance-enhancing substances for the Tour de France, most notably EPO, during the 1999 tour. Armstrong was later stripped of his seven tour wins by USADA, and the UCI concurred with the decision, even though several of his wins occurred outside of the 8 year statute of limitations. Tour organizers have removed Armstrong's name and results from the race's history. These severe penalties are a direct result of the findings outlined in USADAs "Reasoned Decision" which goes beyond Armstrong's personal cheating to outline how he and team manager, Johan Bruyneel, forced other cyclists to dope as well. The document goes to the root of their doping network, also targeting the shadowy, doctors and back room enablers who helped cyclists procure and administer drugs and highly placed executives who helped to avoid doping controls and hide positive test results.[42]

Witnesses testified that code words used for EPO included "Edgar", "Poe",[43] "Edgar Allen Poe", and "Zumo" (Spanish for 'juice').[44]

History[edit]

In 1905, Paul Carnot, a professor of medicine in Paris, and his assistant, Clotilde Deflandre, proposed the idea that hormones regulate the production of red blood cells. After conducting experiments on rabbits subject to bloodletting, Carnot and Deflandre attributed an increase in red blood cells in rabbit subjects to a hemotropic factor called hemopoietin. Eva Bonsdorff and Eeva Jalavisto continued to study red cell production and later called the hemopoietic substance 'erythropoietin'. Further studies investigating the existence of EPO by K.R. Reissman (unknown location) and Allan J. Erslev (Thomas Jefferson Medical College) demonstrated that a certain substance, circulated in the blood, is able to stimulate red blood cell production and increase hematocrit. This substance was finally purified and confirmed as erythropoietin, opening doors to therapeutic uses for EPO in diseases such as anemia.[16][45]

Haematologist John Adamson and nephrologist Joseph W. Eschbach looked at various forms of renal failure and the role of the natural hormone EPO in the formation of red blood cells. Studying sheep and other animals in the 1970s, the two scientists helped establish that EPO stimulates the production of red cells in bone marrow and could lead to a treatment for anemia in humans. In 1968, Goldwasser and Kung began work to purify human EPO, and managed to purify milligram quantities of over 95% pure material by 1977.[46] Pure EPO allowed the amino acid sequence to be partially identified and the gene to be isolated.[16] Later, an NIH-funded researcher at Columbia University discovered a way to synthesize EPO. Columbia University patented the technique, and licensed it to Amgen. Controversy has ensued over the fairness of the rewards that Amgen reaped from NIH-funded work, and Goldwasser was never financially rewarded for his work.[47]

In the 1980s, Adamson, Joseph W. Eschbach, Joan C. Egrie, Michael R. Downing and Jeffrey K. Browne conducted a clinical trial at the Northwest Kidney Centers for a synthetic form of the hormone, Epogen, produced by Amgen. The trial was successful, and the results were published in the New England Journal of Medicine in January 1987.[48]

In 1985, Lin et al isolated the human erythropoietin gene from a genomic phage library and were able to characterize it for research and production.[49] Their research demonstrated the gene for erythropoietin encoded the production of EPO in mammalian cells that is biologically active in vitro and in vivo. The industrial production of recombinant human erythropoietin (RhEpo) for treating anemia patients would begin soon after.

In 1989, the US Food and Drug Administration approved the hormone Epogen, which remains in use today.

See also[edit]

References[edit]

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  2. ^ Haroon ZA, Amin K, Jiang X, Arcasoy MO (September 2003). "A novel role for erythropoietin during fibrin-induced wound-healing response". Am. J. Pathol. 163 (3): 993–1000. doi:10.1016/S0002-9440(10)63459-1. PMC 1868246. PMID 12937140. 
  3. ^ a b "Safety Labeling Changes: Epogen/Procrit (epoetin alfa) and Aranesp (darbepoetin alfa)". MedWatch: The FDA Safety Information and Adverse Event Reporting Program. United States Food and Drug Administration. 2011-08-11. 
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Further reading[edit]

  • Takeuchi M, Kobata A (1992). "Structures and functional roles of the sugar chains of human erythropoietins". Glycobiology 1 (4): 337–46. doi:10.1093/glycob/1.4.337. PMID 1820196. 
  • Semba RD, Juul SE (2002). "Erythropoietin in human milk: physiology and role in infant health". Journal of human lactation : official journal of International Lactation Consultant Association 18 (3): 252–61. PMID 12192960. 
  • Ratcliffe PJ (2003). "From erythropoietin to oxygen: hypoxia-inducible factor hydroxylases and the hypoxia signal pathway". Blood Purif. 20 (5): 445–50. doi:10.1159/000065201. PMID 12207089. 
  • Westenfelder C (2002). "Unexpected renal actions of erythropoietin". Exp. Nephrol. 10 (5–6): 294–8. doi:10.1159/000065304. PMID 12381912. 
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  • Goldwasser, Eugene. A Bloody Long Journey: Erythropoietin and the Person Who Isolated It. Xlibris, 2011. ISBN 978-1-4568-5737-0

External links[edit]


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297 news items

Nature World News

Nature World News
Wed, 27 Aug 2014 00:30:00 -0700

Erythropoietin - a hormone used by athletes to boost performance - can help prevent brain damage in preterm babies. Scientists at the Faculty of Medicine of the University of Geneva (UNIGE) and the University Hospital of Geneva (HUG) say that three ...

BBC News

2 Minute Medicine
Wed, 27 Aug 2014 03:30:00 -0700

1. The administration of high dose recombinant erythropoietin to premature infants within 42 hours after birth was associated with a decreased risk of brain injury. 2. Analysis of the primary outcome of this study assessing brain development at 2 years ...

The Pharmaceutical Journal

The Pharmaceutical Journal
Fri, 29 Aug 2014 06:41:15 -0700

Preliminary findings from the Swiss EPO Neuroprotection Trial found that preterm infants given three doses of recombinant human erythropoietin just before and just after birth were significantly less likely to have brain abnormalities on magnetic ...

BBC News

International Association of Athletics Federations
Mon, 01 Sep 2014 01:34:02 -0700

Erythropoiesis-Stimulating Agents [e.g. erythropoietin (EPO), darbepoetin (dEPO), hypoxia-inducible factor (HIF) stabilizers and activators (e.g. xenon, argon), methoxy polyethylene glycol-epoetin beta (CERA), peginesatide (Hematide)]. The revised 2014 ...
 
Healio
Wed, 27 Aug 2014 07:03:45 -0700

Two frequently used anemia treatments did not improve short-term neurocognitive outcomes in patients who experienced a traumatic brain injury, according to results of a randomized trial. Neither treatment with erythropoietin nor maintaining hemoglobin ...

Headlines & Global News

Headlines & Global News
Fri, 29 Aug 2014 08:11:15 -0700

Erythropoietin is a type of hormone that helps the body create more red blood cells. At least 400,000 children were born prematurely in Europe and these babies showed great risks in having brain damage, according to the World Health Organization ...

Tech Times

Tech Times
Wed, 27 Aug 2014 04:03:45 -0700

The hormone erythropoietin, or EPO, which is illegally used to boost athlete performance, appears to have beneficial effects on very premature babies. Findings of a new study suggest that the hormone could protect pre-term infants from brain damage ...
 
News-Medical.net
Tue, 26 Aug 2014 23:11:15 -0700

High-dose erythropoietin (EPO; a hormone) administered within 42 hours of birth to preterm infants was associated with a reduced risk of brain injury, as indicated by magnetic resonance imaging, according to a study in the August 27 issue of JAMA ...
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